叶酸脂质体包裹的MYCN siRNA在裸鼠体内靶向治疗作用的初步研究(3)
综上所述，叶酸脂质体MYCN siRNA在裸鼠体内靶向肿瘤组织，叶酸脂质体是肿瘤基因治疗的有效载体。叶酸脂质体MYCN siRNA在体内显著下调肿瘤MYCN mRNA的表达，促进肿瘤细胞凋亡，有望成为神经母细胞瘤治疗的新药物。
[参考文献]
[1] 冯晨，唐锁勤，王建文，等.以叶酸受体为靶向的脂质体介导MYCN基因小干扰RNA体外抑制神经母细胞瘤LA-N-5细胞的增值[J].军医进修学院学报，2010，31(2)：161-163.
[2] Pearson A，Lunec J. The MYCN oncoprotein as a drug development target [J]. Cancer Lett，1997，(3)：125-130.
[3] Nara K，Kusafuka T，Yoneda A，et al. Silencing of MYCN by RNA interference induces growth inhibition，apoptotic activity and cell differentiation in a neuroblastoma cell line with MYCN amplification [J]. Int J Oncol，2007，30(5)：1189-1196.
[4] Huang Y，Hong J，Zheng S，et al. Elimination pathways of systemically delivered siRNA [J]. Mol Ther，2011，19(2)：381-385.
[5] Birmingham A，Anderson EM，Reynolds A，et al. 3'' UTR seed matches, but not overall identity, are associated with RNAi off-targets [J]. Nat Methods，2006，3：199-204.
[6] Stevens PJ，Sekido M，Lee RJ. A folate receptor-targeted lipid nanoparticle formulation for a lipophilic paclitaxel prodrug [J]. Pharm Res，2004，21(12)：2153-2157.
[7] Daldrup HE，Hawkins RA，Meier R，et al. Receptor imaging of pediatric tumors： clinical practice and new developments [J]. Pediatr Radiol，2008，38(11)：1154-1161.
[8] Pan X，Lee RJ. Tumour-selective drug delivery via folate receptor-targeted liposomes [J]. Expert Opin Drug Deliv，2004，1(1)：7-17.
(收稿日期：2012-04-20 本文编辑：卫轲), http://www.100md.com(朱琪琦 于芳 冯晨 唐锁勤)
[参考文献]
[1] 冯晨，唐锁勤，王建文，等.以叶酸受体为靶向的脂质体介导MYCN基因小干扰RNA体外抑制神经母细胞瘤LA-N-5细胞的增值[J].军医进修学院学报，2010，31(2)：161-163.
[2] Pearson A，Lunec J. The MYCN oncoprotein as a drug development target [J]. Cancer Lett，1997，(3)：125-130.
[3] Nara K，Kusafuka T，Yoneda A，et al. Silencing of MYCN by RNA interference induces growth inhibition，apoptotic activity and cell differentiation in a neuroblastoma cell line with MYCN amplification [J]. Int J Oncol，2007，30(5)：1189-1196.
[4] Huang Y，Hong J，Zheng S，et al. Elimination pathways of systemically delivered siRNA [J]. Mol Ther，2011，19(2)：381-385.
[5] Birmingham A，Anderson EM，Reynolds A，et al. 3'' UTR seed matches, but not overall identity, are associated with RNAi off-targets [J]. Nat Methods，2006，3：199-204.
[6] Stevens PJ，Sekido M，Lee RJ. A folate receptor-targeted lipid nanoparticle formulation for a lipophilic paclitaxel prodrug [J]. Pharm Res，2004，21(12)：2153-2157.
[7] Daldrup HE，Hawkins RA，Meier R，et al. Receptor imaging of pediatric tumors： clinical practice and new developments [J]. Pediatr Radiol，2008，38(11)：1154-1161.
[8] Pan X，Lee RJ. Tumour-selective drug delivery via folate receptor-targeted liposomes [J]. Expert Opin Drug Deliv，2004，1(1)：7-17.
(收稿日期：2012-04-20 本文编辑：卫轲), http://www.100md.com(朱琪琦 于芳 冯晨 唐锁勤)