卵母细胞体外成熟培养液的改良及研究(1)
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[摘要] 目的 探讨卵母细胞体外成熟培养液的改良及研究。 方法 选取昆明小鼠50只作为研究对象，根据培养液的不同将50只小鼠分为A组和B组，每组各25只。A组采用低糖细胞培养基(DMEM)培养，B组采用含15%～20%胎牛血清的人类输卵管液培养，两组在相同条件下进行培养，比较两组的体外受精率、正常受精率、卵裂率、优质胚胎率、可移植胚胎率、妊娠率及活产率。 [关键词] 卵母细胞体外成熟；培养液；小鼠；体外受精
[中图分类号] R711.6 [文献标识码] A [文章编号] 1673-7210(2014)04(b)-0020-04
[Abstract] Objective To explore the improvement and research of the cultured medium in in-vitro maturation. Methods 50 Kunming mice were selected as the research objects， they were divided into group A and group B according to the different cultured medium， each group of 25 mice. Group A was given low sugar dulbecco's modified eagle medium (DMEM) culture， group B was given human tube fluid containing 15%-20% fetal bovine serum ......
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