高效液相色谱—蒸发光散射检测器法测定中药茺蔚子中盐酸水苏碱的含量(1)
[摘要] 目的 建立中药茺蔚子中盐酸水苏碱的高效液相色谱-蒸发光散射检测器测定方法。 方法 Asahipak NH2P 50 4E色谱柱(4.6 mm × 250 mm)，流动相为乙腈-0.2%磷酸溶液(84∶16)，体积流量1 mL/min，柱温30℃。 结果 盐酸水苏碱在0.487～4.383 μg范围内线性关系良好，r = 0.9999，平均回收率为97.96%，RSD为0.92%。 结论 该法灵敏度高，专属性强，可作为中药茺蔚子中盐酸水苏碱的定量测定方法。
[关键词] 茺蔚子；盐酸水苏碱；高效液相色谱-蒸发光散射检测器法
[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2015)05(c)-0124-04
[Abstract] Objective To establish HPLC-ELSD determination method of the hydrochloric acid stachydrine in Motherwort Fruit. Methods The separation by HPLC-ELSD was performed on Asahipak NH2P 50 4E column (4.6 mm × 250 mm) with acetonitrile-0.2% phosphoric acid solution (84∶16) as mobile phase ......
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[关键词] 茺蔚子；盐酸水苏碱；高效液相色谱-蒸发光散射检测器法
[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2015)05(c)-0124-04
[Abstract] Objective To establish HPLC-ELSD determination method of the hydrochloric acid stachydrine in Motherwort Fruit. Methods The separation by HPLC-ELSD was performed on Asahipak NH2P 50 4E column (4.6 mm × 250 mm) with acetonitrile-0.2% phosphoric acid solution (84∶16) as mobile phase ......
您现在查看是摘要页，全文长 3896 字符。