微囊化肝细胞移植代偿肝功能的实验研究(1)
[摘要] 目的 探讨微囊化肝细胞移植对肝脏功能的代偿能力。 方法 以D-氨基半乳糖胺(D-gal)作为肝脏毒剂，构建SD大鼠急性肝功能衰竭模型。通过腹腔移植分别植入微囊化肝细胞和游离肝细胞。结合谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、总胆红素(TBIL)多项血生化指标以及模型动物生存率比较，评估植入细胞对肝功能衰竭的代偿能力。 结果 细胞植入后12 h开始对模型动物的肝生化指标ALT、AST、TBIL、ALB产生影响，其影响力在细胞植入后24～48 h达到高峰。对比游离肝细胞，微囊化肝细胞移植对各项肝生化指标的改善尤为明显，且动物的存活率最高。 结论 微囊化肝细胞腹腔内移植有助于提高药物诱导急性肝功能衰竭大鼠的存活率，可明显改善急性肝功能衰竭模型大鼠的肝功能。
[关键词] 肝细胞；微囊化；移植；肝衰竭
[中图分类号] R318 [文献标识码] A [文章编号] 1673-7210(2016)01(a)-0004-05
Experimental compensatory ability of hepatic failure by transplantation of microencapsulated hepatocytes
SUN Lixia1 LIU Dawei2 LIU Fang2 FAN Xinjuan2 ZHAO Guoqiang2
1.Department of Pathology， Jiangmen Central Hospital Affiliated to Sun Yat-Sen University， Guangdong Province， Jiangmen 529030， China； 2.Department of Pathology， the First Affiliated Hospital of Sun Yat-Sen University， Guangdong Province， Guangzhou 510080， China
[Abstract] Objective To investigate the compensatory ability of microencapsulated hepatocyte transplantation on acute hepatic failure rats. Methods An acute hepatic failure rat model was induced by celiac injection of D-gal. Microencapsulated and non-microencapsulated rat hepatocytes BRL were transplanted into acute hepatic failure model rat by abdominal cavity administration， respectively. The blood levels of ALT， AST， TBIL， ALB as well as survival rate of host rats were comparably analyzed to evaluate the compensatory ability of microencapsulated hepatocytes for the prostrate liver. Results Levels of ALT， AST， TBIL and ALB were influenced in acute hepatic failure rat model at 12 h after transplantation， and peaked between 24-48 h. Compared with the non-microencapsulated cell， the level of liver biochemical index improved obviously and the survival rate was the highest in microencapsulated hepatocytes. Conclusion After microencapsulated hepatocyte transplantation， the survival rate of acute liver failure rats induced by D-gal increased and the liver function improved obviously.
[Key words] Hepatocyte； Microencapsulated； Transplantation； Hepatic failure
由各种急性肝损害引起的急性肝功能衰竭(acute liver failure，ALF)严重危及人类生命。临床上肝功能重建的途径主要有同位肝移植、生物人工肝技术以及肝细胞移植。肝细胞移植技术前景十分诱人，但因细胞来源复杂，宿主对移植物的免疫排斥反应等问题遭遇发展瓶颈。微囊化包裹肝细胞技术为肝细胞大规模及高活性体外培养提供了新的途径。本实验采用D-氨基半乳糖诱导大鼠急性肝衰竭模型，经腹腔内移植微囊化的大鼠肝细胞，通过观察分析模型动物肝脏多项生化指标的变化特征、肝组织的病理学变化以及模型动物的存活率，评估植入细胞对肝功能衰竭的代偿能力。
1 材料与方法
1.1 仪器与试剂
无菌超净台(苏州超净仪器设备厂)、细胞培养箱(Precision，美国)、细胞培养瓶(Corning 美国)、倒置相差显微镜、冷场发射扫描电镜(日本电子株式会社，由中山大学测试中心提供)、全自动生化分析仪(日本日立7170型)、JB-2型恒温磁力搅拌器(上海电磁新泾仪器有限公司)、VORTEX-GENIE2振荡器(Scienlific Industries)、微囊制备仪(自制)、D-氨基半乳糖胺(江苏启东九丰工贸有限公司)、DMEM/F12干粉(美国Gibico公司)、海藻酸钠(国药集团化学试剂有限公司)、壳聚糖(浙江澳兴生物技术有限公司)。, 百拇医药(孙丽霞 刘大伟 刘芳 范新娟 赵国强)
[关键词] 肝细胞；微囊化；移植；肝衰竭
[中图分类号] R318 [文献标识码] A [文章编号] 1673-7210(2016)01(a)-0004-05
Experimental compensatory ability of hepatic failure by transplantation of microencapsulated hepatocytes
SUN Lixia1 LIU Dawei2 LIU Fang2 FAN Xinjuan2 ZHAO Guoqiang2
1.Department of Pathology， Jiangmen Central Hospital Affiliated to Sun Yat-Sen University， Guangdong Province， Jiangmen 529030， China； 2.Department of Pathology， the First Affiliated Hospital of Sun Yat-Sen University， Guangdong Province， Guangzhou 510080， China
[Abstract] Objective To investigate the compensatory ability of microencapsulated hepatocyte transplantation on acute hepatic failure rats. Methods An acute hepatic failure rat model was induced by celiac injection of D-gal. Microencapsulated and non-microencapsulated rat hepatocytes BRL were transplanted into acute hepatic failure model rat by abdominal cavity administration， respectively. The blood levels of ALT， AST， TBIL， ALB as well as survival rate of host rats were comparably analyzed to evaluate the compensatory ability of microencapsulated hepatocytes for the prostrate liver. Results Levels of ALT， AST， TBIL and ALB were influenced in acute hepatic failure rat model at 12 h after transplantation， and peaked between 24-48 h. Compared with the non-microencapsulated cell， the level of liver biochemical index improved obviously and the survival rate was the highest in microencapsulated hepatocytes. Conclusion After microencapsulated hepatocyte transplantation， the survival rate of acute liver failure rats induced by D-gal increased and the liver function improved obviously.
[Key words] Hepatocyte； Microencapsulated； Transplantation； Hepatic failure
由各种急性肝损害引起的急性肝功能衰竭(acute liver failure，ALF)严重危及人类生命。临床上肝功能重建的途径主要有同位肝移植、生物人工肝技术以及肝细胞移植。肝细胞移植技术前景十分诱人，但因细胞来源复杂，宿主对移植物的免疫排斥反应等问题遭遇发展瓶颈。微囊化包裹肝细胞技术为肝细胞大规模及高活性体外培养提供了新的途径。本实验采用D-氨基半乳糖诱导大鼠急性肝衰竭模型，经腹腔内移植微囊化的大鼠肝细胞，通过观察分析模型动物肝脏多项生化指标的变化特征、肝组织的病理学变化以及模型动物的存活率，评估植入细胞对肝功能衰竭的代偿能力。
1 材料与方法
1.1 仪器与试剂
无菌超净台(苏州超净仪器设备厂)、细胞培养箱(Precision，美国)、细胞培养瓶(Corning 美国)、倒置相差显微镜、冷场发射扫描电镜(日本电子株式会社，由中山大学测试中心提供)、全自动生化分析仪(日本日立7170型)、JB-2型恒温磁力搅拌器(上海电磁新泾仪器有限公司)、VORTEX-GENIE2振荡器(Scienlific Industries)、微囊制备仪(自制)、D-氨基半乳糖胺(江苏启东九丰工贸有限公司)、DMEM/F12干粉(美国Gibico公司)、海藻酸钠(国药集团化学试剂有限公司)、壳聚糖(浙江澳兴生物技术有限公司)。, 百拇医药(孙丽霞 刘大伟 刘芳 范新娟 赵国强)