芒柄花黄素对大鼠局部脑缺血再灌注损伤保护作用的初步研究(1)
[摘要] 目的 探讨芒柄花黄素对大鼠脑局部缺血再灌注损伤的神经保护作用。 方法 采用随机数字表法将42只SD雄性大鼠分为假手术组、缺血再灌注组和芒柄花黄素组，每组14只。芒柄花黄素组腹腔注射芒柄花黄素溶液20 mg/kg，假手术组和缺血再灌注组注射等剂量生理盐水，连续7 d。线栓法复制大鼠大脑中动脉缺血再灌注模型，缺血时间1.5 h。再灌注后24 h各组分别进行神经功能缺损测定；干/湿重法检测脑水肿程度；2，3，5-氯化三苯基四氮唑(TTC)染色计算脑梗死体积并检测血浆超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平。 结果 与缺血再灌注组比较，芒柄花黄素组大鼠神经功能缺损评分降低，第二脑片梗死体积和第三脑片水肿程度减轻，血浆SOD活性提高，MDA水平下降，差异均有统计学意义(P < 0.05或P < 0.01)。 结论 芒柄花黄素可改善大鼠脑缺血再灌注后神经功能缺损程度，减小脑梗死体积，其机制可能与减低氧化应激水平有关。
[关键词] 芒柄花黄素；脑缺血再灌注损伤；植物雌激素；神经功能缺损
[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210(2017)11(b)-0008-04
[Abstract] Objective To investigate the protective effect of formononetin on local cerebral ischemia-reperfusion injury in rats. Methods According to the random number table method， 42 male SD rats were divided into sham group， ischemia-reperfusion group and formononetin group， with 14 rats in each group. The formononetin group was given formononetin solution 20 mg/kg by intraperitoneal injection， the sham group and ischemia-reperfusion group were injected with same dose of normal saline， for 7 days. Suture-occluded method was used to copy the model of rats with cerebral ischemia-reperfusion injury in middle cerebral artery， the ischemic time was 1.5 h. After reperfusion for 24 h， the neurologic impairment of all groups was detected； the dry/wet method was used to detect the degree of cerebral edema； 2，3，5-tripheyltetrazolium (TTC) staining was used to calculate the volume of cerebral infarction and detect the activities of plasma superoxide dismutase (SOD) and the levels of malondialdehyde (MDA). Results Compared with ischemia-reperfusion group， the score of neurologic impairment in formononetin group was decreased， the volume of cerebral infarction of the second brain slice and the degree of cerebral edema of the third brain slice were reduced， the activities of plasma SOD were increased， and the levels of MDA were declined， the differences were all statistically significant (P < 0.05 or P < 0.01). Conclusion Formononetin can improve the degree of neurologic impairment， reduce the volume of cerebral infarction， the mechanism may be related to the decrease of oxidative stress.
[Key words] Formononetin； Cerebral ischemia-reperfusion injury； Phytoestrogen； Neurologic impairment
雌激素對脑缺血再灌注损伤有保护作用，但雌激素替代治疗可能增加乳腺癌和子宫癌的发病率，这使雌激素的应用受到争议[1-2]。植物雌激素被认为性质更为温和，副作用小于雌激素。芒柄花黄素(formononetin)是黄芪、鸡血藤、红三叶草等多种中药的有效成分之一，其异黄酮结构具有植物雌激素(phytoestrogen)作用。本研究通过线栓法复制大鼠局部脑缺血再灌注模型，探讨芒柄花黄素是否能够降低大鼠神经损伤和脑梗死程度，起到神经保护作用。
1 材料与方法, http://www.100md.com(姜慈静 吴秋慧 任倩瑶 王勇 陈健 )
[关键词] 芒柄花黄素；脑缺血再灌注损伤；植物雌激素；神经功能缺损
[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210(2017)11(b)-0008-04
[Abstract] Objective To investigate the protective effect of formononetin on local cerebral ischemia-reperfusion injury in rats. Methods According to the random number table method， 42 male SD rats were divided into sham group， ischemia-reperfusion group and formononetin group， with 14 rats in each group. The formononetin group was given formononetin solution 20 mg/kg by intraperitoneal injection， the sham group and ischemia-reperfusion group were injected with same dose of normal saline， for 7 days. Suture-occluded method was used to copy the model of rats with cerebral ischemia-reperfusion injury in middle cerebral artery， the ischemic time was 1.5 h. After reperfusion for 24 h， the neurologic impairment of all groups was detected； the dry/wet method was used to detect the degree of cerebral edema； 2，3，5-tripheyltetrazolium (TTC) staining was used to calculate the volume of cerebral infarction and detect the activities of plasma superoxide dismutase (SOD) and the levels of malondialdehyde (MDA). Results Compared with ischemia-reperfusion group， the score of neurologic impairment in formononetin group was decreased， the volume of cerebral infarction of the second brain slice and the degree of cerebral edema of the third brain slice were reduced， the activities of plasma SOD were increased， and the levels of MDA were declined， the differences were all statistically significant (P < 0.05 or P < 0.01). Conclusion Formononetin can improve the degree of neurologic impairment， reduce the volume of cerebral infarction， the mechanism may be related to the decrease of oxidative stress.
[Key words] Formononetin； Cerebral ischemia-reperfusion injury； Phytoestrogen； Neurologic impairment
雌激素對脑缺血再灌注损伤有保护作用，但雌激素替代治疗可能增加乳腺癌和子宫癌的发病率，这使雌激素的应用受到争议[1-2]。植物雌激素被认为性质更为温和，副作用小于雌激素。芒柄花黄素(formononetin)是黄芪、鸡血藤、红三叶草等多种中药的有效成分之一，其异黄酮结构具有植物雌激素(phytoestrogen)作用。本研究通过线栓法复制大鼠局部脑缺血再灌注模型，探讨芒柄花黄素是否能够降低大鼠神经损伤和脑梗死程度，起到神经保护作用。
1 材料与方法, http://www.100md.com(姜慈静 吴秋慧 任倩瑶 王勇 陈健 )