炎症相关性结直肠癌小鼠模型建立及血管生成抑制因子1在结直肠癌中的表达(1)
[摘要] 目的 探讨炎症相关性结直肠癌小鼠模型的建立，及血管生成抑制因子1(VASH-1)在结直肠癌中的表达。 方法 将雄性小鼠(C57BL/6)30只按体重大小随机分为两组。A组给予氧化偶氮甲烷(AOM)10 mg/kg右侧腹腔注射，后于第1、4、7周给予1.5%～2.5%葡聚糖硫酸钠(DSS)自由饮用7 d，其余时间自由饮清水。B组给予生理盐水0.1 mL/10 g右侧腹腔注射，此后给予清水自由饮用。均在第10周末处死小鼠，查看成瘤情况。免疫组织化学染色法探讨VASH-1在结直肠癌组织中的表达。 结果 造模结束后A组成瘤率为53.33%，病理提示为腺癌，B组成瘤率为0.00%，两组成瘤率比较差异有统计学意义(P < 0.05)。VASH-1在结直肠癌中高表达，在正常结直肠组织中低表达，差异有统计学意义(P < 0.05)。 结论 本方法建立的炎症相关性结直肠癌小鼠模型成瘤率较高。VASH-1在结直肠癌组织中的表达高于正常结直肠组织。
[关键词] 炎症性肠病；结直肠肿瘤；动物模型；血管生成抑制因子
[中图分类号] R735.3 [文献标识码] A [文章编号] 1673-7210(2017)12(c)-0017-04
[Abstract] Objective To investigate the establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 (VASH-1) in colorectal cancer. Methods Thirty male mice (C57BL/6) were randomly divided into two groups according to the body weight. Group A was given right intraperitoneal administration of azoxymethane (AOM) 10 mg/kg first， and free drink of 1.5%-2.5% dextran sulfate sodium (DSS) at the first， fourth， seventh week later for 7 d， which drunk distilled water on the other time. Group B was given right intraperitoneal administration of normal saline 0.1 mL/10 g， then the mice were given distilled water on the other time. The mice of both groups were killed at the end of tenth week to observe the conditions of tumor formation. The immunohistochemical staining was performed to evaluate the expression of VASH-1 in the of tissue colorectal cancer. Results After modeling， the tumor formation rate of group A was 53.33%， with the pathologic findings of adenocarcinoma， which of group B was 0.00%， the difference was statistically significant (P < 0.05). VASH-1 had high expression in the colorectal cancer and low expression in normal colorectal tissues， with significant difference (P < 0.05). Conclusion The tumor formation rate of mice model of inflammation-associated colorectal cancer induced by this method is high. The expression of VASH-1 in colorectal cancer is higher than that in normal colorectal tissues.
[Key words] Inflammatory bowel disease； Colorectal cancer； Animal model； Vasohibin
結直肠癌是常见的恶性肿瘤，结直肠癌的发生与多种因素有关，包括遗传[1]、生活习惯及高脂饮食等原因[2]，此外炎症性肠病是引起结直肠癌的另一类原因[3]。结直肠癌的动物模型可模拟结直肠癌的多种生物学特征，是研究结直肠癌发病机制及治疗的重要手段。炎症性肠病引起的结直肠癌常用的动物模型造模方法为氧化偶氮甲烷(azoxymethane，AOM)腹腔注射，之后自由饮用葡聚糖硫酸钠(dextran sulfate sodium，DSS)溶液[4]，文献报道的关于自由饮用DSS的浓度及造模时间多种多样，但应用有效的DSS及缩短造模时间仍在进一步研究中。肿瘤的生长及转移需要依赖肿瘤血管的生成。有些抗肿瘤药物通过抑制肿瘤血管的生成来抑制肿瘤的生长及转移，而在肿瘤血管生成中血管生成抑制因子1(vasohibin-1，VASH-1)是血管生成抑制因子中的一种。本研究探讨炎症相关性结直肠癌小鼠模型的建立及VASH-1在炎症相关性结直肠癌小鼠中的表达情况，或许可以找到抗肿瘤治疗的新靶点。, http://www.100md.com(王磊 时军利 李炳庆)
[关键词] 炎症性肠病；结直肠肿瘤；动物模型；血管生成抑制因子
[中图分类号] R735.3 [文献标识码] A [文章编号] 1673-7210(2017)12(c)-0017-04
[Abstract] Objective To investigate the establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 (VASH-1) in colorectal cancer. Methods Thirty male mice (C57BL/6) were randomly divided into two groups according to the body weight. Group A was given right intraperitoneal administration of azoxymethane (AOM) 10 mg/kg first， and free drink of 1.5%-2.5% dextran sulfate sodium (DSS) at the first， fourth， seventh week later for 7 d， which drunk distilled water on the other time. Group B was given right intraperitoneal administration of normal saline 0.1 mL/10 g， then the mice were given distilled water on the other time. The mice of both groups were killed at the end of tenth week to observe the conditions of tumor formation. The immunohistochemical staining was performed to evaluate the expression of VASH-1 in the of tissue colorectal cancer. Results After modeling， the tumor formation rate of group A was 53.33%， with the pathologic findings of adenocarcinoma， which of group B was 0.00%， the difference was statistically significant (P < 0.05). VASH-1 had high expression in the colorectal cancer and low expression in normal colorectal tissues， with significant difference (P < 0.05). Conclusion The tumor formation rate of mice model of inflammation-associated colorectal cancer induced by this method is high. The expression of VASH-1 in colorectal cancer is higher than that in normal colorectal tissues.
[Key words] Inflammatory bowel disease； Colorectal cancer； Animal model； Vasohibin
結直肠癌是常见的恶性肿瘤，结直肠癌的发生与多种因素有关，包括遗传[1]、生活习惯及高脂饮食等原因[2]，此外炎症性肠病是引起结直肠癌的另一类原因[3]。结直肠癌的动物模型可模拟结直肠癌的多种生物学特征，是研究结直肠癌发病机制及治疗的重要手段。炎症性肠病引起的结直肠癌常用的动物模型造模方法为氧化偶氮甲烷(azoxymethane，AOM)腹腔注射，之后自由饮用葡聚糖硫酸钠(dextran sulfate sodium，DSS)溶液[4]，文献报道的关于自由饮用DSS的浓度及造模时间多种多样，但应用有效的DSS及缩短造模时间仍在进一步研究中。肿瘤的生长及转移需要依赖肿瘤血管的生成。有些抗肿瘤药物通过抑制肿瘤血管的生成来抑制肿瘤的生长及转移，而在肿瘤血管生成中血管生成抑制因子1(vasohibin-1，VASH-1)是血管生成抑制因子中的一种。本研究探讨炎症相关性结直肠癌小鼠模型的建立及VASH-1在炎症相关性结直肠癌小鼠中的表达情况，或许可以找到抗肿瘤治疗的新靶点。, http://www.100md.com(王磊 时军利 李炳庆)