血清中针对TORCH相关病原的IgG和IgM抗体分析(1)
[摘要] 目的 通過分析孕前、妊娠早期妇女和新生患儿三组人群血清中TORCH[弓形虫(TOX)、风疹病毒(RV)、巨细胞病毒(CMV)、单纯疱疹病毒(HSV1/2)]抗体水平，为TORCH检测在优生优育中的合理应用提供理论指导。 方法 收集2014年1月～2017年12月于昆明市第一人民医院行孕前检查(孕前组，1929份)、孕早期检查(妊娠早期组，15 555份)、新出生即入院治疗患儿(新生患儿组，2049份)血清样本共计19 533份。采用酶联免疫吸附法(ELISA)对三组人群血清中TORCH病原体的IgG和IgM抗体水平进行检测和分析。 结果 TORCH抗体的ELISA分析结果显示，19 533份样品中TORCH病原抗体IgG阳性率为99.41%，其中CMV的IgG抗体阳性率最高(97.27%)，TOX的最低(3.73%)。IgM阳性率为12.80%，其中针对HSV1的最高(6.44%)，而CMV的最低(0.39%)。进一步分组分析发现，孕前组、妊娠早期组和新生患儿组血清样品中TORCH相关病原IgG抗体总阳性率均在99%以上。孕前组和妊娠早期组血清中TORCH相关病原IgM抗体阳性率分别为18.87%和13.65%，高于新生患儿组(0.63%)。1例患者同时携带3种TORCH病原IgG抗体的占大多数(>60%)，而携带IgM抗体的主要只是针对一种TORCH病原。 结论 TORCH相关病原对育龄妇女普遍易感，育龄妇女孕前、孕早期、新出生患儿均进行TORCH血清IgG和IgM抗体筛查，对于TORCH病原感染预防控制、促进优生优育具有十分重要的指导意义。
[关键词] TORCH；感染；血清抗体；酶联免疫吸附法；优生优育
[中图分类号] R714.251 [文献标识码] A [文章编号] 1673-7210(2018)04(b)-0085-05
[Abstract] Objective To provide theoretical guidelines for reasonable application of TORCH assay in aristogenesis and fine rearing through analysis of serum antibody of IgG and IgM against TORCH-associated pathogens (TOX， RV， CMV， HSV1/2) from the pre-pregnant， pregnant patients at early stage and new-born patients. Methods From January of 2014 to December of 2017， 19 533 serum samples were collected from pre-pregnant (pre-pregnant group， 1929 samples)， pregnant patients at early stage (pregnant group， 15 555 samples) and new-born patients (new-born group， 2049 samples) in the First People's Hospital of Kunming. ELISA was performed to detect the levels of antibodies IgG and IgM against TORCH-associated pathogens and further determine positive rates of specific antibodies of IgG and IgM. Results ELISA of TORCH antibodies in 19 533 serum samples showed that the positive rate of antibody IgG against any of TORCH-pathogens reached 99.41%. The positive rate of CMV IgG (97.27%) was the highest among four kinds of TORCH-associated pathogens and TOX (3.7%) showed the lowest rate. The positive rate of IgM antibody was 12.8%. The positive rate of HSV1 (6.44%) was the highest and CMV showed the lowest rate (0.39%). Further analysis of the positive rate of IgG by groups showed the more than 99% of IgG positive rate of TORCH-associated pathogens in all groups of pre-pregnant group， pregnant group at early stage and new born group. The IgM positive rates of TORCH-associated pathogens in pre-pregnant group and pregnant group at early stage， 18.87% and 13.65%， were higher than that in new-born group (0.63%). There were more than 60% of individual serum sample showed the IgG antibodies against 3 TORCH-associated pathogens. However， most of the IgM antibody in individual serum sample was against one of TORCH-associated pathogens. Conclusion The pre-pregnant， pregnant women at early stage and new-born patients are screened for antibodies of IgG and IgM in serum against TORCH-associated pathogens， which is of instructive significance for controlling infections of TORCH pathogens and aristogenesis and fine rearing., http://www.100md.com(杨悦 林罗纯 胡大春)
[关键词] TORCH；感染；血清抗体；酶联免疫吸附法；优生优育
[中图分类号] R714.251 [文献标识码] A [文章编号] 1673-7210(2018)04(b)-0085-05
[Abstract] Objective To provide theoretical guidelines for reasonable application of TORCH assay in aristogenesis and fine rearing through analysis of serum antibody of IgG and IgM against TORCH-associated pathogens (TOX， RV， CMV， HSV1/2) from the pre-pregnant， pregnant patients at early stage and new-born patients. Methods From January of 2014 to December of 2017， 19 533 serum samples were collected from pre-pregnant (pre-pregnant group， 1929 samples)， pregnant patients at early stage (pregnant group， 15 555 samples) and new-born patients (new-born group， 2049 samples) in the First People's Hospital of Kunming. ELISA was performed to detect the levels of antibodies IgG and IgM against TORCH-associated pathogens and further determine positive rates of specific antibodies of IgG and IgM. Results ELISA of TORCH antibodies in 19 533 serum samples showed that the positive rate of antibody IgG against any of TORCH-pathogens reached 99.41%. The positive rate of CMV IgG (97.27%) was the highest among four kinds of TORCH-associated pathogens and TOX (3.7%) showed the lowest rate. The positive rate of IgM antibody was 12.8%. The positive rate of HSV1 (6.44%) was the highest and CMV showed the lowest rate (0.39%). Further analysis of the positive rate of IgG by groups showed the more than 99% of IgG positive rate of TORCH-associated pathogens in all groups of pre-pregnant group， pregnant group at early stage and new born group. The IgM positive rates of TORCH-associated pathogens in pre-pregnant group and pregnant group at early stage， 18.87% and 13.65%， were higher than that in new-born group (0.63%). There were more than 60% of individual serum sample showed the IgG antibodies against 3 TORCH-associated pathogens. However， most of the IgM antibody in individual serum sample was against one of TORCH-associated pathogens. Conclusion The pre-pregnant， pregnant women at early stage and new-born patients are screened for antibodies of IgG and IgM in serum against TORCH-associated pathogens， which is of instructive significance for controlling infections of TORCH pathogens and aristogenesis and fine rearing., http://www.100md.com(杨悦 林罗纯 胡大春)