通天草醇提物对阿尔茨海默病小鼠行为学及海马炎症介质的影响(1)
[摘要] 目的 探討通天草醇提物对阿尔茨海默病小鼠学习能力、记忆能力、学习记忆保持能力等行为学及海马炎症介质白细胞介素(IL)-1β、肿瘤坏死因子α(TNF-α)含量的影响。 方法 根据随机数字表法将48只雄性C57BL/6小鼠分为六组：模型组、正常组、多奈哌齐组和通天草醇提物高、中、低剂量组，每组8只。采用Aβ1-42(80 pmol/μL)双侧脑室注射的方法诱导阿尔茨海默病模型，各组实验开始每天灌胃1次，多奈哌齐组小鼠给予0.000 92 g/(kg·d)灌胃，通天草醇提物高、中、低剂量组小鼠分别给予3.96、1.98、0.99 g/(kg·d)灌胃，而正常组和模型组小鼠给予生理盐水灌胃，连续治疗35 d。采用Morris水迷宫检测阿尔茨海默病小鼠行为学的改变，酶联免疫吸附法检测阿尔茨海默病小鼠海马IL-1β、TNF-α含量。 结果 模型组小鼠的逃避潜伏期及游泳距离增加，同时伴有目标停留时间缩短和跨平台次数减少，寻找到水迷宫系统水下平台所需要的时间较长，与正常组比较，模型组小鼠行为学出现明显异常(P < 0.05)；与正常组比较，模型组海马炎症介质IL-1β、TNF-α水平明显升高，差异有统计学意义(P < 0.05)；与模型组比较，通天草醇提物高、中剂量组小鼠行为学明显改善，海马IL-1β、TNF-α水平明显降低，差异有统计学意义(P < 0.05)。 结论 通天草醇提物能够改善小鼠的学习能力和记忆障碍，降低炎性反应并缓解血脑屏障损伤，在阿尔茨海默病治疗中发挥重要作用。
[关键词] 通天草；阿尔茨海默病；行为学；炎症介质
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210(2018)09(a)-0017-04
[Abstract] Objective To investigate the effects of alcohol extract of Waternut Herb on learning ability， memory ability， learning and memory retention ability of mice with Alzheimer disease， and the effects of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in hippocampal inflammation media. Methods Forty-eight male C57BL/6 mice were divided into six groups according to the random number table method： model group， normal group， Donepezil group， and high， medium， low dose of alcohol extract of Waternut Herb group， with 8 mice in each group. Alzheimer disease model was induced by bilateral intracere broventricular injection of Aβ1-42 (80 pmol/μL). Each group of rats was given intragastric administration once a day. The mice in Donepezil group were given 0.000 92 g/(kg·d) by gavage. The mice in the high， medium， low dose of alcohol extract of Waternut Herb group were given 3.96， 1.98， 0.99 g/(kg·d) by gavage， respectively. While the mice in the normal group and model group were given intragastric administration with continuous saline， treatment for 35 days. The Morris water maze was used to detect the changes of behavior in Alzheimer disease mice. The levels of IL-1β and TNF-α in hippocampus of Alzheimer disease mice were determined by enzyme-linked immunosorbent assay. Results The incubation period and swimming distance of mice in the model group were increased. At the same time， the target residence time was shortened and the number of cross-platforms was reduced， it took a long time to find the underwater platform of the water maze system， compared with the normal group， the abnormal behavior in the model group was significantly increased (P < 0.05). Compared with the normal group， the levels of IL-1β and TNF-α of the inflammatory mediators of the hippocampus in model group were significantly increased (P < 0.05). Compared with the model group， the behavior of mice in the high and medium doses of alcohol extract of Waternut Herb group was significantly improved， and the levels of IL-1β and TNF-α in the hippocampus were significantly decreased， the differences were statistically significant (P < 0.05). Conclusion Alcohol extract of Waternut Herb can improve the learning ability and memory impairment of mice， reduce the inflammatory response and relieve blood-brain barrier injury， and plays an important role in the treatment of Alzheimer disease., http://www.100md.com(仲丽丽 张维嘉 刘旭)
[关键词] 通天草；阿尔茨海默病；行为学；炎症介质
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210(2018)09(a)-0017-04
[Abstract] Objective To investigate the effects of alcohol extract of Waternut Herb on learning ability， memory ability， learning and memory retention ability of mice with Alzheimer disease， and the effects of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in hippocampal inflammation media. Methods Forty-eight male C57BL/6 mice were divided into six groups according to the random number table method： model group， normal group， Donepezil group， and high， medium， low dose of alcohol extract of Waternut Herb group， with 8 mice in each group. Alzheimer disease model was induced by bilateral intracere broventricular injection of Aβ1-42 (80 pmol/μL). Each group of rats was given intragastric administration once a day. The mice in Donepezil group were given 0.000 92 g/(kg·d) by gavage. The mice in the high， medium， low dose of alcohol extract of Waternut Herb group were given 3.96， 1.98， 0.99 g/(kg·d) by gavage， respectively. While the mice in the normal group and model group were given intragastric administration with continuous saline， treatment for 35 days. The Morris water maze was used to detect the changes of behavior in Alzheimer disease mice. The levels of IL-1β and TNF-α in hippocampus of Alzheimer disease mice were determined by enzyme-linked immunosorbent assay. Results The incubation period and swimming distance of mice in the model group were increased. At the same time， the target residence time was shortened and the number of cross-platforms was reduced， it took a long time to find the underwater platform of the water maze system， compared with the normal group， the abnormal behavior in the model group was significantly increased (P < 0.05). Compared with the normal group， the levels of IL-1β and TNF-α of the inflammatory mediators of the hippocampus in model group were significantly increased (P < 0.05). Compared with the model group， the behavior of mice in the high and medium doses of alcohol extract of Waternut Herb group was significantly improved， and the levels of IL-1β and TNF-α in the hippocampus were significantly decreased， the differences were statistically significant (P < 0.05). Conclusion Alcohol extract of Waternut Herb can improve the learning ability and memory impairment of mice， reduce the inflammatory response and relieve blood-brain barrier injury， and plays an important role in the treatment of Alzheimer disease., http://www.100md.com(仲丽丽 张维嘉 刘旭)