D-双功能蛋白在子宫内膜癌组织中的表达及意义(1)
[摘要] 目的 檢测子宫内膜癌组织中D-双功能蛋白(DBP)表达水平，及其与血清肿瘤标志物癌胚抗原(CEA)、糖类抗原125(CA125)检测结果的相关性，探讨DBP对子宫内膜癌诊断和治疗的临床意义。 方法 选取2013年1月～2015年12月在河北省唐山市妇幼保健院(以下简称“我院”)进行子宫内膜癌切除术患者30例作为实验组，选取我院同期健康体检的正常者20名作为对照组，应用化学发光法检查血清肿瘤标志物CEA和CA125的表达水平，免疫组织化学法检测子宫内膜癌组织和癌旁组织DBP的表达水平，测量积分光密度值(IOD)，采用Pearson相关分析法分析DBP与肿瘤标志物CEA、CA125的相关性。 结果 子宫内膜癌组织中DBP的IOD值表达高于癌旁组织，差异有统计学意义(P < 0.05)，且DBP与肿瘤标志物CEA(r = 0.837，P < 0.01)和CA125(r = 0.787，P < 0.01)的表达水平呈线性正相关。 结论 子宫内膜癌患者DBP的表达升高，并且与子宫内膜癌的发生发展密切相关。CEA、CA125和DBP联合检测对子宫内膜癌早期诊断有重要的意义，并为子宫内膜癌的治疗和预后提供了一个新的研究靶点。
[关键词] 子宫内膜癌;D-双功能蛋白;癌胚抗原;糖类抗原125;基因诊断
[中图分类号] R737.33 [文献标识码] A [文章编号] 1673-7210(2019)02(a)-0161-04
[Abstract] Objective To detect the expression level of D-bifunctional protein (DBP) in endometrial cancer tissues and its correlation with the detection results of serum tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125)， and to explore the clinical significance of DBP in the diagnosis and treatment of endometrial cancer. Methods From January 2013 to December 2015， 30 cases of patients undergoing endometrial cancer resection in Tangshan Maternal and Child Health Care Hospital (hereinafter referred to as "our hospital") were selected as the experimental group， and 20 cases of normal persons who underwent physical examination during the same period in our hospital were selected as the control group. Chemiluminescence was used to detect the expression levels of serum tumor markers CEA and CA125， and immunohistochemistry was used to detect the expression levels of DBP in endometrial and paracancer tissues， and the integral optical density value (IOD) was measured. Pearson correlation analysis was used to analyze the correlation between DBP and tumor markers CEA and CA125. Results The expression of IOD value of DBP in endometrial carcinoma was higher than that in adjacent tissues， and the difference was statistically significant (P < 0.05). There was a positive linear correlation between DBP and tumor markers CEA (r = 0.837， P < 0.01) and CA125 (r = 0.787， P < 0.01). Conclusion The expression of DBP is increased in patients with endometrial cancer and is closely related to the occurrence and development of endometrial cancer. The combined detection of CEA， CA125 and DBP has important significance for the early diagnosis of endometrial cancer， and provides a new research target for the treatment and prognosis of endometrial cancer., 百拇医药(路欣 孔令玉)
[关键词] 子宫内膜癌;D-双功能蛋白;癌胚抗原;糖类抗原125;基因诊断
[中图分类号] R737.33 [文献标识码] A [文章编号] 1673-7210(2019)02(a)-0161-04
[Abstract] Objective To detect the expression level of D-bifunctional protein (DBP) in endometrial cancer tissues and its correlation with the detection results of serum tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125)， and to explore the clinical significance of DBP in the diagnosis and treatment of endometrial cancer. Methods From January 2013 to December 2015， 30 cases of patients undergoing endometrial cancer resection in Tangshan Maternal and Child Health Care Hospital (hereinafter referred to as "our hospital") were selected as the experimental group， and 20 cases of normal persons who underwent physical examination during the same period in our hospital were selected as the control group. Chemiluminescence was used to detect the expression levels of serum tumor markers CEA and CA125， and immunohistochemistry was used to detect the expression levels of DBP in endometrial and paracancer tissues， and the integral optical density value (IOD) was measured. Pearson correlation analysis was used to analyze the correlation between DBP and tumor markers CEA and CA125. Results The expression of IOD value of DBP in endometrial carcinoma was higher than that in adjacent tissues， and the difference was statistically significant (P < 0.05). There was a positive linear correlation between DBP and tumor markers CEA (r = 0.837， P < 0.01) and CA125 (r = 0.787， P < 0.01). Conclusion The expression of DBP is increased in patients with endometrial cancer and is closely related to the occurrence and development of endometrial cancer. The combined detection of CEA， CA125 and DBP has important significance for the early diagnosis of endometrial cancer， and provides a new research target for the treatment and prognosis of endometrial cancer., 百拇医药(路欣 孔令玉)