依达拉奉对大鼠脑缺血再灌注损伤的机制研究(1)
[摘要] 目的 探讨依达拉奉对脑缺血再灌注大鼠PDCD-5蛋白表达的影响。 方法 将体重为250～280 g的健康雄性SD大鼠78只按照随机数字表法分为空白对照组(n = 6，生理盐水，3 mg/kg，2次/d，腹腔内注射)、假手术组(n = 24，生理盐水，3 mg/kg，2次/d，腹腔内注射)、脑缺血再灌注组(n = 24，生理盐水，3 mg/kg，2次/d，腹腔内注射)及药物干预组(n = 24，依达拉奉3 mg/kg，2次/d，腹腔内注射)，后三组再随机分为6 h组、1 d组、3 d组、7 d组。选择改良Longa法建立脑缺血再灌注模型。比较各组神经功能缺损情况、脑梗死面积及PDCD-5和Caspase-3蛋白在不同时期海马组织中的表达。 结果 脑缺血再灌注组脑梗死灶较假手术组明显增加(P < 0.05)，药物干预组脑梗死灶较脑缺血再灌注组明显减少(P < 0.05)。同时，不同时间点脑缺血再灌注组的PDCD-5、Caspase-3蛋白表達均高于假手术组和药物干预组，差异均有统计学意义(P < 0.05)。 结论 依达拉奉对脑缺血再灌注大鼠海马组织中PDCD-5的表达起抑制作用，并对大鼠脑缺血再灌注损伤起脑保护作用。
[关键词] 脑缺血再灌注；PDCD-5；依达拉奉；大鼠
[中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210(2019)05(a)-0016-04
Study on the mechanism of Edaravone on cerebral ischemia-reperfusion injury in rats
MI Yan1 GAO Xiaoping2 ZHU Qingfeng2 LUO Hailong3
1.Department of Electrophysiology， Hu′nan Thoracic Hospital， Hu′nan Province， Changsha 410000， China； 2.Department of Neurology， Hu′nan People′s Hospital， Hu′nan Province， Changsha 410000， China； 3.Department of Endoscopic Diagnosis， Hu′nan Thoracic Hospital， Hu′nan Province， Changsha 410000， China
[Abstract] Objective To investigate the effect of Edaravone on the expression of PDCD-5 protein in rats with cerebral ischemia reperfusion. Methods Seventy-eight healthy male SD rats with a healthy weight of 250-280 g were randomly divided into blank control group (n = 6， normal saline， 3 mg/kg， twice a day， intraperitoneal injection)， sham operation group (n = 24， normal saline， 3 mg/kg， twice a day， intraperitoneal injection)， cerebral ischemia reperfusion group (n = 24， normal saline， 3 mg/kg， twice a day， intraperitoneal injection) and drug intervention group (n = 24， Edaravone 3 mg/kg， twice a day， intraperitoneal injection) according to random number table method， and the latter 3 groups were randomly divided into 6 h group， 1 d group， 3 d group and 7 d group. A modified Longa method was used to establish cerebral ischemia-reperfusion model. The neurological deficit， the acreage of cerebral infarction and the expression of PDCD-5 and Caspase-3 protein in hippocampus of different periods were compared. Results The cerebral infarction in cerebral ischemia reperfusion group was significantly increased compared with sham operation group (P < 0.05)； the cerebral infarction in drug intervention group was significantly reduced compared with cerebral ischemia reperfusion group (P < 0.05). At the same time， the expression of PDCD-5， Caspase-3 protein in cerebral ischemia-reperfusion group at different time points was higher than those in corresponding sham-operation group and corresponding drug intervention group (P < 0.05). Conclusion Edaravone can inhibit the expression of PDCD-5 in the hippocampus of rats with cerebral ischemia-reperfusion， and protect the brain against cerebral ischemia-reperfusion injury in rats., 百拇医药(米艳 高小平 朱清风)
[关键词] 脑缺血再灌注；PDCD-5；依达拉奉；大鼠
[中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210(2019)05(a)-0016-04
Study on the mechanism of Edaravone on cerebral ischemia-reperfusion injury in rats
MI Yan1 GAO Xiaoping2 ZHU Qingfeng2 LUO Hailong3
1.Department of Electrophysiology， Hu′nan Thoracic Hospital， Hu′nan Province， Changsha 410000， China； 2.Department of Neurology， Hu′nan People′s Hospital， Hu′nan Province， Changsha 410000， China； 3.Department of Endoscopic Diagnosis， Hu′nan Thoracic Hospital， Hu′nan Province， Changsha 410000， China
[Abstract] Objective To investigate the effect of Edaravone on the expression of PDCD-5 protein in rats with cerebral ischemia reperfusion. Methods Seventy-eight healthy male SD rats with a healthy weight of 250-280 g were randomly divided into blank control group (n = 6， normal saline， 3 mg/kg， twice a day， intraperitoneal injection)， sham operation group (n = 24， normal saline， 3 mg/kg， twice a day， intraperitoneal injection)， cerebral ischemia reperfusion group (n = 24， normal saline， 3 mg/kg， twice a day， intraperitoneal injection) and drug intervention group (n = 24， Edaravone 3 mg/kg， twice a day， intraperitoneal injection) according to random number table method， and the latter 3 groups were randomly divided into 6 h group， 1 d group， 3 d group and 7 d group. A modified Longa method was used to establish cerebral ischemia-reperfusion model. The neurological deficit， the acreage of cerebral infarction and the expression of PDCD-5 and Caspase-3 protein in hippocampus of different periods were compared. Results The cerebral infarction in cerebral ischemia reperfusion group was significantly increased compared with sham operation group (P < 0.05)； the cerebral infarction in drug intervention group was significantly reduced compared with cerebral ischemia reperfusion group (P < 0.05). At the same time， the expression of PDCD-5， Caspase-3 protein in cerebral ischemia-reperfusion group at different time points was higher than those in corresponding sham-operation group and corresponding drug intervention group (P < 0.05). Conclusion Edaravone can inhibit the expression of PDCD-5 in the hippocampus of rats with cerebral ischemia-reperfusion， and protect the brain against cerebral ischemia-reperfusion injury in rats., 百拇医药(米艳 高小平 朱清风)