联合检测血清NF-κB、TNF-α水平对tPSA灰区前列腺癌患者的诊断价值
[摘要] 目的 研究前列腺癌(PCa)患者血清中腫瘤坏死因子-α(TNF-α)及核因子κB(NF-κB)的诊断价值。 方法 选取2015年1月～2018年1月广西科技大学附属柳州市人民医院(以下简称“我院”)诊治的PCa患者100例作为PCa组，选取我院同期诊治的良性前列腺增生(BPH)患者40例为BPH组，选取我院同期体检的健康人群40名为健康对照组。应用电化学发光法检测各组血清总PSA(tPSA)水平，酶联免疫吸附试验(ELISA)检测各组血清TNF-α和NF-κB水平，受试者工作曲线(ROC)分析各指标在PCa中的诊断价值。 结果 BPH组与PCa组患者血清tPSA比较，差异有统计学意义(P < 0.05)。PCa组TNF-α、NF-κB水平高于健康对照组、BPH组，差异均有统计学意义(均P < 0.05)。PCa组患者TNF-α、NF-κB表达与病理分级、Gleason评分及肿瘤分期有关(均P < 0.05)，与年龄、初始tPSA及是否存在远处转移无关(P > 0.05)。TNF-α、NF-κB及联合检测的敏感性分别为75.1%、79.2%及81.3%，特异性分别为71.5%、80.1%及82.4%。ROC曲线的曲线下面积分别为0.713、0.753及0.832，联合诊断的诊断效能大于任一单一指标的诊断效能。 结论 PCa患者血清TNF-α、NF-κB表达升高，联合检测患者血清TNF-α、NF-κB对tPSA处于诊断灰区的PCa有较高的诊断价值。
[关键词] 前列腺癌;前列腺特异抗原;核因子-κB;肿瘤坏死因子-α
[中图分类号] R737.25 [文献标识码] A [文章编号] 1673-7210(2020)01(c)-0065-05
Diagnostic value of combined detection of serum NF-κB， TNF-α levels in patients with tPSA gray area prostate cancer
ZHOU Yi WANG Wei LIU Jie YAO Yuan YANG Jianwen
Deparment of Urology Surgery， Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology， Guangxi Zhuang Autonomous Region， Liuzhou 545060， China
[Abstract] Objective To investigate the diagnostic value of tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in serum of prostate cancer (PCa) patients. Methods From January 2015 to January 2018， 100 cases of PCa diagnosed and treated in Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology (hereinafter referred to as “our hospital”) were selected as the PCa group. Forty patients with benign prostatic hyperplasia (BPH) diagnosed and treated in our hospital during the same period were selected as the BPH group， 40 healthy subjects who underwent physical examination in our hospital during the same period were selected as the healthy control group. Determination of total PSA (tPSA) in each group by electrochemical luminescence. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum TNF-α and NF-κB in each group， and the differences in the expression of each indicator were statistically analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of each indicator in PCa. Results Comparison of serum tPSA between the BPH group and the PCa group showed statistically significant difference (P < 0.05). The levels of TNF-α and NF-κB in the PCa group were higher than those in the healthy control group and the BPH group， with statistically significant differences (all P < 0.05). The expression of TNF-α and NF-κB in the PCa group was correlated with pathological grade， Gleason score and tumor stage (all P < 0.05)， and was independent of age， initial tPSA and the presence of distant metastasis (P > 0.05). Sensitivity and specificity of TNF-α， NF-κB and combined assays were 75.1%， 79.2% and 81.3%， respectively， and 71.5%， 80.1% and 82.4%， respectively. The area under the ROC curve was 0.713， 0.753 and 0.832， respectively. The diagnostic efficiency of joint diagnosis was higher than that of any single indicator. Conclusion The expressions of TNF-α and NF-κB in serum of PCa patients were increased， and the combined detection of TNF-α and NF-κB in serum of PCa patients was of high diagnostic value for PCa in the gray area of diagnosis., 百拇医药(周毅 王伟 刘杰 姚远 杨剑文)
[关键词] 前列腺癌;前列腺特异抗原;核因子-κB;肿瘤坏死因子-α
[中图分类号] R737.25 [文献标识码] A [文章编号] 1673-7210(2020)01(c)-0065-05
Diagnostic value of combined detection of serum NF-κB， TNF-α levels in patients with tPSA gray area prostate cancer
ZHOU Yi WANG Wei LIU Jie YAO Yuan YANG Jianwen
Deparment of Urology Surgery， Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology， Guangxi Zhuang Autonomous Region， Liuzhou 545060， China
[Abstract] Objective To investigate the diagnostic value of tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in serum of prostate cancer (PCa) patients. Methods From January 2015 to January 2018， 100 cases of PCa diagnosed and treated in Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology (hereinafter referred to as “our hospital”) were selected as the PCa group. Forty patients with benign prostatic hyperplasia (BPH) diagnosed and treated in our hospital during the same period were selected as the BPH group， 40 healthy subjects who underwent physical examination in our hospital during the same period were selected as the healthy control group. Determination of total PSA (tPSA) in each group by electrochemical luminescence. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum TNF-α and NF-κB in each group， and the differences in the expression of each indicator were statistically analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of each indicator in PCa. Results Comparison of serum tPSA between the BPH group and the PCa group showed statistically significant difference (P < 0.05). The levels of TNF-α and NF-κB in the PCa group were higher than those in the healthy control group and the BPH group， with statistically significant differences (all P < 0.05). The expression of TNF-α and NF-κB in the PCa group was correlated with pathological grade， Gleason score and tumor stage (all P < 0.05)， and was independent of age， initial tPSA and the presence of distant metastasis (P > 0.05). Sensitivity and specificity of TNF-α， NF-κB and combined assays were 75.1%， 79.2% and 81.3%， respectively， and 71.5%， 80.1% and 82.4%， respectively. The area under the ROC curve was 0.713， 0.753 and 0.832， respectively. The diagnostic efficiency of joint diagnosis was higher than that of any single indicator. Conclusion The expressions of TNF-α and NF-κB in serum of PCa patients were increased， and the combined detection of TNF-α and NF-κB in serum of PCa patients was of high diagnostic value for PCa in the gray area of diagnosis., 百拇医药(周毅 王伟 刘杰 姚远 杨剑文)