NF-κBIA基因多态性与新生儿细菌性脑膜炎易感性相关性分析(1)
[摘要] 目的 探究NF-κBIA基因多态性与新生儿细菌性脑膜炎易感性的关系。 方法 选取2016年3月～2019年3月南方医科大学附属深圳妇幼保健院新生儿科收治的新生儿211例，其中新生儿脑膜炎患者67例为病例组，正常新生儿69名为对照组，非脑膜炎炎性发热新生儿75例为发热组。采用Sanger法测序，通过PCR扩增，对NF-κBIA基因的2个SNP位点(rs3138053、rs2233406)进行基因分型。观察NF-κBIA基因多态性与新生儿细菌性脑膜炎的易感相关性。 结果 三组新生儿rs3138053、rs2233406位点的期望值和观察值吻合度良好，均符合哈迪温伯格平衡(P > 0.05)。rs3138053位点的基因型频率与新生儿细菌型脑膜炎易感性存在相关性(P < 0.05)。 结论 NF-κBIA基因多态性与新生儿细菌性脑膜炎易感性相关。
[关键词] 新生儿细菌性脑膜炎;NF-κBIA基因;单核苷酸多态性;基因型
[中图分类号] R742.6 [文献标识码] A [文章编号] 1673-7210(2020)02(c)-0019-04
[Abstract] Objective To investigate the relationship between NF-κBIA gene polymorphism and neonatal bacterial meningitis susceptibility. Methods From March 2016 to March 2019， 211 cases admitted to the Department of Neonatology， Affiliated Shenzhen Maternity & Child Healthcare Hospital， Southern Medical University. Among them， 67 cases of neonatal meningitis were in the case group， 69 normal neonates were in the control group， and 75 cases with non-meningitis inflammatory febrile neonates were in the fever group. Two SNP sites (rs3138053 and rs2233406) of NF-κBIA gene were genotyped by PCR amplification using Sanger sequencing. The genotype sequencing results of different sites and the susceptibility analysis of neonates bacterial meningitis were observed. Results rs3138053 and rs2233406 were in good agreement with the expected value and the observed value in three groups， which were consistent with Hardy-Weinberg equilibrium (P > 0.05). rs3138053 was correlated with the susceptibility of neonatal bacterial meningitis (P < 0.05). Conclusion NF-κBIA gene polymorphism is associated with susceptibility to neonatal bacterial meningitis.
[Key words] Neonatal bacterial meningitis; NF-κBIA gene; Single nucleotide polymorphism; Genotype
新生兒脑膜炎是一种以感染引起的脑膜炎症为特征的疾病，通常发生在生命的前28 d。新生儿细菌性脑膜炎(neonatal bacterial meningitis，NBM)的发病率为(0.25～1)/1000活产婴儿，其中大约25%的新生儿患有菌血症[1]，其病死率和后遗症发生率高[2]。核因子-κB(NF-κB)是新近研究发现的一组经典炎症信号通路中与炎性反应相关的基因，研究发现NF-κB基因的表达与细菌性脑膜炎的发生关系密切， 由NF-κBIA编码的IkBa是NF-κB活性的重要抑制剂[3-4]。单核苷酸多态性(SNP)是人类遗传学变异中最常见的一种。目前尚没有NF-κBIA基因多态性与新生儿细菌性脑膜炎相关研究，本研究旨在探讨新生儿NF-κBIA基因多态性与新生儿细菌性脑膜炎易感性的关系。
1 资料与方法
1.1 一般资料
采用病例-对照研究方法，收集2016年3月～2019年3月南方医科大学附属深圳妇幼保健院(以下简称“我院”)新生儿科住院的足月新生儿，其中脑膜炎新生儿67例为病例组，正常新生儿69名为对照组，非脑膜炎炎性发热[5]新生儿75例为发热组。对照组，男27例，女42例;孕龄37～41周，平均(39.1±1.2)周;出生体重2900～3900 g，平均(3333.2±437.9)g。发热组，男32例，女43例;孕龄37～41周，平均(38.8±1.6)周;出生体重2900～3900 g，平均(3324.3±547.1)g。病例组男26例，女41例;孕龄37～41周，平均(38.7±1.6)周;出生体重2900～3900 g，平均(3243.5±678.1)g。三组一般资料比较，差异无统计学差异(P > 0.05)，具有可比性。本研究经我院医学伦理委员会批准，参与研究患儿家属均签署知情同意。, 百拇医药(李文娟 梅家平 杨勇)
[关键词] 新生儿细菌性脑膜炎;NF-κBIA基因;单核苷酸多态性;基因型
[中图分类号] R742.6 [文献标识码] A [文章编号] 1673-7210(2020)02(c)-0019-04
[Abstract] Objective To investigate the relationship between NF-κBIA gene polymorphism and neonatal bacterial meningitis susceptibility. Methods From March 2016 to March 2019， 211 cases admitted to the Department of Neonatology， Affiliated Shenzhen Maternity & Child Healthcare Hospital， Southern Medical University. Among them， 67 cases of neonatal meningitis were in the case group， 69 normal neonates were in the control group， and 75 cases with non-meningitis inflammatory febrile neonates were in the fever group. Two SNP sites (rs3138053 and rs2233406) of NF-κBIA gene were genotyped by PCR amplification using Sanger sequencing. The genotype sequencing results of different sites and the susceptibility analysis of neonates bacterial meningitis were observed. Results rs3138053 and rs2233406 were in good agreement with the expected value and the observed value in three groups， which were consistent with Hardy-Weinberg equilibrium (P > 0.05). rs3138053 was correlated with the susceptibility of neonatal bacterial meningitis (P < 0.05). Conclusion NF-κBIA gene polymorphism is associated with susceptibility to neonatal bacterial meningitis.
[Key words] Neonatal bacterial meningitis; NF-κBIA gene; Single nucleotide polymorphism; Genotype
新生兒脑膜炎是一种以感染引起的脑膜炎症为特征的疾病，通常发生在生命的前28 d。新生儿细菌性脑膜炎(neonatal bacterial meningitis，NBM)的发病率为(0.25～1)/1000活产婴儿，其中大约25%的新生儿患有菌血症[1]，其病死率和后遗症发生率高[2]。核因子-κB(NF-κB)是新近研究发现的一组经典炎症信号通路中与炎性反应相关的基因，研究发现NF-κB基因的表达与细菌性脑膜炎的发生关系密切， 由NF-κBIA编码的IkBa是NF-κB活性的重要抑制剂[3-4]。单核苷酸多态性(SNP)是人类遗传学变异中最常见的一种。目前尚没有NF-κBIA基因多态性与新生儿细菌性脑膜炎相关研究，本研究旨在探讨新生儿NF-κBIA基因多态性与新生儿细菌性脑膜炎易感性的关系。
1 资料与方法
1.1 一般资料
采用病例-对照研究方法，收集2016年3月～2019年3月南方医科大学附属深圳妇幼保健院(以下简称“我院”)新生儿科住院的足月新生儿，其中脑膜炎新生儿67例为病例组，正常新生儿69名为对照组，非脑膜炎炎性发热[5]新生儿75例为发热组。对照组，男27例，女42例;孕龄37～41周，平均(39.1±1.2)周;出生体重2900～3900 g，平均(3333.2±437.9)g。发热组，男32例，女43例;孕龄37～41周，平均(38.8±1.6)周;出生体重2900～3900 g，平均(3324.3±547.1)g。病例组男26例，女41例;孕龄37～41周，平均(38.7±1.6)周;出生体重2900～3900 g，平均(3243.5±678.1)g。三组一般资料比较，差异无统计学差异(P > 0.05)，具有可比性。本研究经我院医学伦理委员会批准，参与研究患儿家属均签署知情同意。, 百拇医药(李文娟 梅家平 杨勇)
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