藏药普尔那不同部位体外抑菌及抗肿瘤活性研究(1)
[摘要] 目的 研究藏药普尔那不同部位不同提取物的体外抗耐药菌和抗肿瘤活性。 方法 对藏药普尔那茎和叶分别采用无水乙醇、乙酸乙酯进行成分提取。采用K-B纸片法、比浊法及平板划线法，评价不同提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌活性。MTT法检测不同提取物对人肝癌细胞系HepG2细胞、人宫颈癌细胞系HeLa细胞的抑制率。 结果 37℃培养18 h后，普尔那叶无水乙醇提取物(100.00 mg/mL)对MRSA(1×107 cfu/mL)抑制作用最强(5.00 mm)，最低抑菌浓度(MIC)为1.25 mg/mL，最低杀菌浓度(MBC)为5 mg/mL。高浓度(500.00 μg/mL)普尔那叶乙酸乙酯提取物对人肝癌细胞系HepG2细胞、人宫颈癌细胞系HeLa细胞均表现出最佳的抑制活性。 结论 普尔那叶乙醇提取物对MRSA体外抑耐药菌效果更佳，而普尔那叶乙酸乙酯提取物抗肿瘤活性更好。
[关键词] 藏药;普尔那;耐甲氧西林金黄色葡萄球菌;抗耐药菌;抗肿瘤
[中图分类号] R291.4 [文献标识码] A [文章编号] 1673-7210(2020)07(b)-0017-04
[Abstract] Objective To study on anti-drug resistant bacteria and antitumor activity of different parts from different parts of Tibetan medicine Artemisia vestita in vitro. Methods Stem and leaf of Artemisia vestita were extracted with absolute ethanol and ethyl acetate respectively. The antibacterial activity of different extracts on Methicillin-resistant Staphylococcus aureus (MRSA) was evaluated by K-B paper method， turbidimetric method and flat line marking method. The MTT method was used to detect the inhibition rate of different extracts on human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells. Results After incubation at 37°C for 18 h， the Artemisia vestita leaf absolute ethanol extract (100.00 mg/mL) had the strongest inhibitory effect (5.00 mm) on MRSA (1×107 cfu/mL)， and the lowest inhibitory concentration (MIC) was 1.25 mg/mL， the minimum germicidal concentration (MBC) was 5 mg/mL. Highconcentration (500.00 μg/mL) Artemisia vestita leaf ethyl acetate extract showed the best inhibitory activity against human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells. Conclusion Artemisia vestita leaf ethanol extract has better anti-drug resistance to MRSA in vitro， while Artemisia vestita leaf ethyl acetate extract has better antitumor activity.
[Key words] Tibetan medicine; Artemisia vestita; Methicillin-resistant Staphylococcus aureus; Anti-drug resistant bacteria; Antitumor
普爾那是菊科植物毛莲蒿的地上部分，味苦、辛，性寒，具有清热解毒、杀虫、止血等功效[1-3]。普尔那中主要含有挥发油、香豆素、有机酸、黄酮、甾体类等多种成分[4-8]，具有抗寄生虫[9-10]、抗炎[11-12]、抗肿瘤[13]、抑菌[14-16]等作用。细菌的耐药问题及肿瘤的高发病率、高死亡率严重威胁人类健康[17-19]。本研究对藏药普尔那茎和叶分别采用无水乙醇、乙酸乙酯进行成分提取，再对提取物进行体外抗耐药菌及抗肿瘤活性研究，从而为促进藏药开发及从天然产物中寻找抗耐药菌、抗肿瘤的药物提供依据。
1 仪器与试药
1.1 仪器
旋转蒸发仪(RE-2000A，上海亚荣生化仪器厂);水浴锅(DF-101SA，瑞力仪器设备源头厂家);十万分之一天平(BS124S，北京赛多利斯仪器系统有限公司);生物安全柜，(BSC-1500ⅡA2-X，济南圣科环保科技有限公司);恒温恒湿培养箱(LHS-250HC-11，上海一恒科学仪器有限公司);压力蒸汽灭菌器(MSG.N 180L，山东威高集团医用高分子制品股份有限公司);数控超声波清洗器(KQ-100DE，昆山市超声仪器有限公司);酶标仪(FC，Labsystems);半径10 cm离心机(H-1600RW，西安超杰仪器设备有限公司);倒置荧光显微镜(IX71，Olympus);高压蒸气灭菌锅(CLG-40M，ALP)。, 百拇医药(李磊 崔鹤蓉 王鹏龙)
[关键词] 藏药;普尔那;耐甲氧西林金黄色葡萄球菌;抗耐药菌;抗肿瘤
[中图分类号] R291.4 [文献标识码] A [文章编号] 1673-7210(2020)07(b)-0017-04
[Abstract] Objective To study on anti-drug resistant bacteria and antitumor activity of different parts from different parts of Tibetan medicine Artemisia vestita in vitro. Methods Stem and leaf of Artemisia vestita were extracted with absolute ethanol and ethyl acetate respectively. The antibacterial activity of different extracts on Methicillin-resistant Staphylococcus aureus (MRSA) was evaluated by K-B paper method， turbidimetric method and flat line marking method. The MTT method was used to detect the inhibition rate of different extracts on human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells. Results After incubation at 37°C for 18 h， the Artemisia vestita leaf absolute ethanol extract (100.00 mg/mL) had the strongest inhibitory effect (5.00 mm) on MRSA (1×107 cfu/mL)， and the lowest inhibitory concentration (MIC) was 1.25 mg/mL， the minimum germicidal concentration (MBC) was 5 mg/mL. Highconcentration (500.00 μg/mL) Artemisia vestita leaf ethyl acetate extract showed the best inhibitory activity against human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells. Conclusion Artemisia vestita leaf ethanol extract has better anti-drug resistance to MRSA in vitro， while Artemisia vestita leaf ethyl acetate extract has better antitumor activity.
[Key words] Tibetan medicine; Artemisia vestita; Methicillin-resistant Staphylococcus aureus; Anti-drug resistant bacteria; Antitumor
普爾那是菊科植物毛莲蒿的地上部分，味苦、辛，性寒，具有清热解毒、杀虫、止血等功效[1-3]。普尔那中主要含有挥发油、香豆素、有机酸、黄酮、甾体类等多种成分[4-8]，具有抗寄生虫[9-10]、抗炎[11-12]、抗肿瘤[13]、抑菌[14-16]等作用。细菌的耐药问题及肿瘤的高发病率、高死亡率严重威胁人类健康[17-19]。本研究对藏药普尔那茎和叶分别采用无水乙醇、乙酸乙酯进行成分提取，再对提取物进行体外抗耐药菌及抗肿瘤活性研究，从而为促进藏药开发及从天然产物中寻找抗耐药菌、抗肿瘤的药物提供依据。
1 仪器与试药
1.1 仪器
旋转蒸发仪(RE-2000A，上海亚荣生化仪器厂);水浴锅(DF-101SA，瑞力仪器设备源头厂家);十万分之一天平(BS124S，北京赛多利斯仪器系统有限公司);生物安全柜，(BSC-1500ⅡA2-X，济南圣科环保科技有限公司);恒温恒湿培养箱(LHS-250HC-11，上海一恒科学仪器有限公司);压力蒸汽灭菌器(MSG.N 180L，山东威高集团医用高分子制品股份有限公司);数控超声波清洗器(KQ-100DE，昆山市超声仪器有限公司);酶标仪(FC，Labsystems);半径10 cm离心机(H-1600RW，西安超杰仪器设备有限公司);倒置荧光显微镜(IX71，Olympus);高压蒸气灭菌锅(CLG-40M，ALP)。, 百拇医药(李磊 崔鹤蓉 王鹏龙)