冬凌草对代谢相关脂肪性肝病脂质代谢的影响(1)
[摘要] 目的 探討冬凌草对大鼠代谢相关脂肪性肝病(MAFLD)脂质代谢相关蛋白表达的影响。 方法 通过高脂饲料喂养大鼠复制代谢相关脂肪性肝病模型，选用30只SD雄性大鼠适应性饲养1 d后，采用随机数字表法分为正常组，模型组，冬凌草低、中、高剂量组，每组6只。正常组以普通饲料喂养，其他组均以高脂饲料喂养。冬凌草低、中、高剂量组每天分别按照50、100 mg/(kg·d)和150 mg/(kg·d)进行灌胃;正常组与模型组每天灌胃与其他实验组等体积的生理盐水。HE染色观察大鼠MAFLD肝组织脂肪变性程度;采用全自动生化仪检测三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖(FBG)、空腹胰岛素(FINS);采用Western blot方法检测过氧化物酶体增殖物激活受体-α(PPAR-α)、固醇调节元件结合蛋白-1c(SREBP-1c)、脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACC)的蛋白表达。 结果 正常组大鼠肝组织显微镜下提示结构清晰及完整，肝小叶结构正常，肝细胞索排列整齐;模型组大鼠肝细胞损伤，可见弥漫性肝细胞脂肪变，并有肝细胞气球样变，汇管区伴有炎症细胞浸润，部分点状肝细胞坏死并有碎屑样坏死;冬凌草高剂量组大鼠肝脏的损伤程度比模型组明显减轻，肝细胞脂肪变性减少，仅少许出现点状坏死，少量脂肪滴空泡，汇管区无明显炎细胞浸润。各组大鼠TC、TG、HDL-C、LDL-C、FBG、FINS水平比较，差异有统计学意义(P < 0.05)。模型组大鼠血清TC、TG、LDL-C、FBG、FINS含量高于正常组，HDL-C含量低于正常组，差异有统计学意义(P < 0.05)。冬凌草低剂量组TG、FINS含量低于模型组，差异有统计学意义(P < 0.05);冬凌草中剂量组TC、TG、LDL-C、FBG、FINS含量低于模型组，且TG、FBG低于冬凌草低剂量组，差异有统计学意义(P < 0.05)。冬凌草高剂量组TC、TG、LDL-C、FBG、FINS含量低于模型组，HDL-C含量高于模型组，且TC、TG、LDL-C、FBG低于冬凌草低剂量组，差异有统计学意义(P < 0.05)。模型组PPAR-α、ACC蛋白表达低于正常组，SREBP-1c、FAS蛋白表达高于正常组，差异有统计学意义(P < 0.05)。冬凌草低剂量组PPAR-α、ACC蛋白表达高于模型组，FAS蛋白表达低于模型组，差异有统计学意义(P < 0.05)。冬凌草中、高剂量组PPAR-α、ACC蛋白表达高于模型组及冬凌草低剂量组，SREBP-1c、FAS蛋白表达低于模型组及冬凌草低剂量组，差异有统计学意义(P < 0.05)。 结论 冬凌草对大鼠MAFLD脂质代谢及相关蛋白表达具有调控作用，为进一步研究MAFLD的发生发展机制奠定了基础。
[关键词] 冬凌草;代谢相关脂肪性肝病;过氧化物酶体增殖物激活受体-α;固醇调节元件结合蛋白-1c;脂肪酸合成酶;乙酰辅酶A羧化酶
[中图分类号] R28 [文献标识码] A [文章编号] 1673-7210(2020)09(a)-0004-06
[Abstract] Objective To investigate the effect of Rabdosia Rubescens on the expression of lipid metabolism-associated proteins in rats with metabolism-associated fatty liver disease (MAFLD). Methods The rats were fed with high-fat diet to replicate the metabolic-related fatty liver disease model. A total of 36 SD male rats were adaptively fed for one day， and they were divided into normal group， model group， and Rabdosia Rubescens low， medium， and high-dose groups by random number table method， each with six animals. The normal group was fed with ordinary feed， and the other groups were fed with high-fat feed. The low， medium， and high-doses of Rabdosia Rubescens were given 50， 100 mg/(kg·d) and 150 mg/(kg·d) per day， respectively; the normal group and the model group were given a gavage with the same volume of normal saline as the other experimental groups every day. HE staining was used to observe the degree of fatty degeneration of rat MAFLD liver tissue; automatic biochemical analyzer was used to detect triacylglycerol (TG)， total cholesterol (TC)， high-density lipoprotein cholesterol (HDL-C)， and low-density lipoprotein cholesterol (LDL- C)， fasting blood glucose (FBG)， fasting insulin (FINS); Western blot method was used to detect the expressions of peroxisome proliferator-activated receptor-α (PPAR-α)， sterol regulatory element binding protein-1c (SREBP-1c)， fatty acid synthase (FAS)， acetyl-CoA carboxylase (ACC) protein. Results The liver tissue of rats in the normal group showed clear and complete structure under microscope， the structure of liver lobules was normal， and the hepatocyte cords were neatly arranged. Hepatocyte damage in the model group showed diffuse hepatocyte steatosis with balloon-like hepatocytes， inflammatory cell infiltration in the portal area， and partial punctate hepatocyte necrosis and detrital necrosis. The degree of liver damage in the Rabdosia Rubescens high-dose group was significantly less than that of the model group. The fatty degeneration of hepatocytes was reduced. There was only a few punctate necrosis， a few fat droplets， and no obvious inflammatory cell infiltration in the portal area. The levels of TC， TG， HDL-C， LDL-C， FBG， and FINS of rats in each group were compared， and the differences were statistically significant (P < 0.05). The levels of serum TC， TG， LDL-C， FBG， and FINS in the model group were higher than those in the normal group， while the level of HDL-C was lower than that in the normal group， and the differences were statistically significant (P < 0.05). The content of TG and FINS in the low-dose Rabdosia Rubescens group were lower than those of the model group， and the differences were statistically significant (P < 0.05); the content of TC， TG， LDL-C， FBG， and FINS in the medium-dose Rabdosia Rubescens group were lower than those of the model group， while TG and FBG were lower than those of the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). The content of TC， TG， LDL-C， FBG， and FINS in the high-dose Rabdosia Rubescens group were lower than those of the model group， while the level of HDL-C was higher than that of the model group， and TC， TG， LDL-C， FBG were lower than those of the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the model group were lower than those of the normal group， while the expressions of SREBP-1c and FAS protein were higher than those of the normal group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the low dose of Rabdosia Rubescens group were higher than those of the model group， while the expression of FAS protein was lower than that of the model group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the medium and high-doses Rabdosia Rubescens group were higher than those of the model group and the low-dose Rabdosia Rubescens group， while the expressions of SREBP-1c and FAS protein were lower than those of the model group and the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). Conclusion Rabdosia Rubescens can regulate lipid metabolism and associated protein expression in rats with MAFLD， which lays a foundation for further study on the mechanism of the occurrence and development of MAFLD., 百拇医药(马文骏 史婷婷 白明辉 陈小伟 杨兴鑫)
[关键词] 冬凌草;代谢相关脂肪性肝病;过氧化物酶体增殖物激活受体-α;固醇调节元件结合蛋白-1c;脂肪酸合成酶;乙酰辅酶A羧化酶
[中图分类号] R28 [文献标识码] A [文章编号] 1673-7210(2020)09(a)-0004-06
[Abstract] Objective To investigate the effect of Rabdosia Rubescens on the expression of lipid metabolism-associated proteins in rats with metabolism-associated fatty liver disease (MAFLD). Methods The rats were fed with high-fat diet to replicate the metabolic-related fatty liver disease model. A total of 36 SD male rats were adaptively fed for one day， and they were divided into normal group， model group， and Rabdosia Rubescens low， medium， and high-dose groups by random number table method， each with six animals. The normal group was fed with ordinary feed， and the other groups were fed with high-fat feed. The low， medium， and high-doses of Rabdosia Rubescens were given 50， 100 mg/(kg·d) and 150 mg/(kg·d) per day， respectively; the normal group and the model group were given a gavage with the same volume of normal saline as the other experimental groups every day. HE staining was used to observe the degree of fatty degeneration of rat MAFLD liver tissue; automatic biochemical analyzer was used to detect triacylglycerol (TG)， total cholesterol (TC)， high-density lipoprotein cholesterol (HDL-C)， and low-density lipoprotein cholesterol (LDL- C)， fasting blood glucose (FBG)， fasting insulin (FINS); Western blot method was used to detect the expressions of peroxisome proliferator-activated receptor-α (PPAR-α)， sterol regulatory element binding protein-1c (SREBP-1c)， fatty acid synthase (FAS)， acetyl-CoA carboxylase (ACC) protein. Results The liver tissue of rats in the normal group showed clear and complete structure under microscope， the structure of liver lobules was normal， and the hepatocyte cords were neatly arranged. Hepatocyte damage in the model group showed diffuse hepatocyte steatosis with balloon-like hepatocytes， inflammatory cell infiltration in the portal area， and partial punctate hepatocyte necrosis and detrital necrosis. The degree of liver damage in the Rabdosia Rubescens high-dose group was significantly less than that of the model group. The fatty degeneration of hepatocytes was reduced. There was only a few punctate necrosis， a few fat droplets， and no obvious inflammatory cell infiltration in the portal area. The levels of TC， TG， HDL-C， LDL-C， FBG， and FINS of rats in each group were compared， and the differences were statistically significant (P < 0.05). The levels of serum TC， TG， LDL-C， FBG， and FINS in the model group were higher than those in the normal group， while the level of HDL-C was lower than that in the normal group， and the differences were statistically significant (P < 0.05). The content of TG and FINS in the low-dose Rabdosia Rubescens group were lower than those of the model group， and the differences were statistically significant (P < 0.05); the content of TC， TG， LDL-C， FBG， and FINS in the medium-dose Rabdosia Rubescens group were lower than those of the model group， while TG and FBG were lower than those of the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). The content of TC， TG， LDL-C， FBG， and FINS in the high-dose Rabdosia Rubescens group were lower than those of the model group， while the level of HDL-C was higher than that of the model group， and TC， TG， LDL-C， FBG were lower than those of the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the model group were lower than those of the normal group， while the expressions of SREBP-1c and FAS protein were higher than those of the normal group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the low dose of Rabdosia Rubescens group were higher than those of the model group， while the expression of FAS protein was lower than that of the model group， and the differences were statistically significant (P < 0.05). The expressions of PPAR-α and ACC protein in the medium and high-doses Rabdosia Rubescens group were higher than those of the model group and the low-dose Rabdosia Rubescens group， while the expressions of SREBP-1c and FAS protein were lower than those of the model group and the low-dose Rabdosia Rubescens group， and the differences were statistically significant (P < 0.05). Conclusion Rabdosia Rubescens can regulate lipid metabolism and associated protein expression in rats with MAFLD， which lays a foundation for further study on the mechanism of the occurrence and development of MAFLD., 百拇医药(马文骏 史婷婷 白明辉 陈小伟 杨兴鑫)