[摘要]致龋性菌斑是龋病发生的始动因子，其形成并生长于口腔这一特殊的生理环境中。因此，对其精密而准确的提取技术是科学研究成功的关键。本文就致龋性菌斑的多种提取技术作一综述。

    [关键词]致龋性菌斑；提取技术；进展

    [中图分类号]R 781[文献标志码]A[doi]10.3969/j.issn.1673-5749.2012.01.014

    Research progress on the techniques of extracting cariogenic plaqueLi Jingwen, Wang Sheng, Hu Tao.(Dept. of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China)

    [Abstract]Cariogenic plaque is the key factor to the occurance of caries, which forms and develops in oral cavity, a special physiological enviroment. Therefore, the precious and accurate technique of extraction is vital for successful research. This article sums up the advantages and disadvantages of various techniques of extracting cariogenic plaque.

    [Key words]cariogenic plaque；techniques of extraction；progress

    牙菌斑是一种典型的口腔生物膜，是龋病和牙周病的致病因子。科学合理的提取技术能保证牙菌斑完整的生物学形态，能够提高研究的真实性、可靠性。菌斑提取技术可分为体内与体外培养提取2大类，本文将对其作一综述。

    1体内提取技术

    1.1染色刮取

    采集牙菌斑前，令受检者2 h以上不进食并除去口内食物残渣，然后在无菌纱托或纱球吸湿及局部隔湿条件下，用生理盐水冲洗采样部位，进行菌斑染色后用无菌探针刮取牙齿唇颊面的菌斑样本，置于还原液内[1]。这种方法主要应用于即时评估牙菌斑的形成情况 ......