一株产槲皮素类色素的银杏内生真菌的研究(英文)
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ABSTRACT:More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo bilobaL..They were cultured in the potato dextrose(PD)liquid medium and one endophytic fungus(Gh01)was screened out having the capaci-ty of producing orange pigment.The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC、This is thefirst report on quercetin glycoside produced by endophytic fungus,The effects of me carbon and nitrogen sourtces,metal ions,inltlal pHand incubation tenaperature on pigmem production of the endophytic fungus were evaluated,The optimal temperature and initial pH forpitment production in PD liquld medium were 28℃and 7.0,respectively,The result of orthogonal trial showed that the most suitablecarbon and nigogen source were 20 g/L glucose and 5g/L peptone respectiVely;1g/L chloridize zinc could increase the yield of pigment,The maximum yield of the pigment was 27.515 g/L alter 120hours'successive culture under the optiraal conditions established.
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