摘要 目的：通过观察IL-2及相关受体蛋白水平的变化情况，探讨艾灸对亚急性衰老大鼠胸腺CD4+T细胞的影响。方法：采用D-半乳糖致亚急性衰老大鼠模型。24只清洁级雄性SD大鼠随机分为：正常组，模型组和艾灸组。艾灸组温和灸双侧肾俞穴5 min/次，共计40 d。采用免疫磁珠法分离胸腺CD4+T细胞，并再用Western Blot方法检测各组大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB的蛋白表达量。结果：模型组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白的表达量较正常组降低，差异有统计学意义(P<0.05)；艾灸组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白表达量升高，与模型组比较差异有统计学意义(P<0.05)。结论：艾灸肾俞穴可上调胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB、I-κB的表达。

    关键词 亚急性衰老；IL-2；IL-2Rα；NF-κB；I-κB；艾灸

    Abstract Objective：To observe the effect of moxibustion on CD4+T cells of thymus in subacute aging rats by recording the changes of IL-2 and related receptor protein levels. Methods：Use D-galactose induced subacute aging rat model. Twenty-four male SD rats were randomly divided into normal group ......