“上火”人群中Activin A、E—selectin的差异表达研究(1)
摘要 目的：通过对“上火”人群中细胞因子激活素A(ActivinA)及E选择素(E-selectin)血清含量的测定以及外周血单个核细胞基因表达水平检测，探讨激活素A及E选择素在“上火”人群发病机制的影响和作用。方法：采集临床患者血液样本并提取血清及外周血单个核细胞。应用酶联免疫吸附试验(ELISA)测定血清中激活素A及E选择素的含量，应用RT-PCR法检测激活素A及E选择素的基因表达水平。结果：“上火”患者血清中activinA、E-selectin的水平以及外周血单个核细胞activinA、E-selectin基因表达水平高于正常平和质人群；观察组外周血中activinA、E-selectin的基因表达水平高于阴虚“上火”人群。结论：“上火”是一种局部的炎性反应，机体内环境在多种因素的作用下激活素A水平升高，影响炎性反应介质的作用或者本身作为一种炎性反应介质活化血管内皮细胞刺激E选择素的分泌与合成，诱导炎性反应，这是“上火”机制之一。
关键词 上火；激活素A；E选择素；炎性反应
Abstract Objective：To study of activin A and E selectin in the pathogenesis of “shanghuo” people and influence effect through the determination of serum content and the level of gene expression in peripheral blood mononuclear cells of activin A or E-selectin who suffered from excessive internal heat. Methods：Clinical lit crowd and gentleness in blood samples was collected and serum and peripheral blood mononuclear cells from blood samples was extracted. By enzyme-linked immune sorbent adsorption assay， the content of serum activin A and E-selectin were determined， and RT-PCR detection was sued to detect gene expression level of activin A and E-selectin. Results：The level of activin A and E-selectin in serum of patients with excessive internal heat was higher than that of normal and healthy people. The level of activin A and E-selectin gene expression in peripheral blood mononuclear cells of patients with internal heat was higher than that of normal and healthy people. The gene expression level of activin A and E-selectin in peripheral blood was higher than that of yin deficiency and heat syndrome. Conclusion：Shanghuo is a local inflammation. Under stress， activin A levels are elevated， effecting inflammatory mediators or itself as a mediator of inflammation activated vascular endothelial cells stimulate E-selectin secretion and synthesis， induced inflammatory reaction and to cause the disorder of lipid metabolism in the body′s physiological balance， causing Shanghuo.
Key Words Shanghuo； Activin A； E selectin； Inflammatory reaction
中图分类号：R285.6文献标识码：Adoi：10.3969/j.issn.1673-7202.2017.12.005
“上火”是指人体阴阳失衡，表现为目赤、口疮、口腔潰疡、牙龈肿痛、咽痛、口干、尿赤、便干等一系列热象的疾病[1]。课题组前期大规模流行病学调查发现，引起“上火”的因素主要有情志、饮食、药物等[2]。“上火”常见实热“上火”与阴虚“上火”2种证型[3]。课题组前期利用细胞因子芯片检测6例阴虚“上火”患者“上火”前后血清细胞因子的变化，结果发现下调的细胞因子有10种，上调的细胞因子有23种，其中发现细胞因子Activin A和E-selectin明显升高，差异有统计学意义(P<0.05)，提示细胞因子在“上火”的发病过程中发挥作用。本研究拟在上述基础上，进一步从细胞分子生物学角度探讨Activin A和E-selectin对2种不同类型上火患者的影响。
1 资料与方法
1.1 一般资料 选取浙江中医药大学第一附属医院招募的临床志愿者48例，随机分为实热组、阴虚组、对照组，每组16例，其中男21例，女27例，平均年龄为38.83岁。分别采集促凝管及抗凝管血液，促凝管提取血清，抗凝管提取单核细胞，3组间年龄及性别比较，差异无统计学意义(P>0.05)。见表1。, http://www.100md.com(汪琴静 徐莉 包洁 季巾君 谢冠群 范永升)
关键词 上火；激活素A；E选择素；炎性反应
Abstract Objective：To study of activin A and E selectin in the pathogenesis of “shanghuo” people and influence effect through the determination of serum content and the level of gene expression in peripheral blood mononuclear cells of activin A or E-selectin who suffered from excessive internal heat. Methods：Clinical lit crowd and gentleness in blood samples was collected and serum and peripheral blood mononuclear cells from blood samples was extracted. By enzyme-linked immune sorbent adsorption assay， the content of serum activin A and E-selectin were determined， and RT-PCR detection was sued to detect gene expression level of activin A and E-selectin. Results：The level of activin A and E-selectin in serum of patients with excessive internal heat was higher than that of normal and healthy people. The level of activin A and E-selectin gene expression in peripheral blood mononuclear cells of patients with internal heat was higher than that of normal and healthy people. The gene expression level of activin A and E-selectin in peripheral blood was higher than that of yin deficiency and heat syndrome. Conclusion：Shanghuo is a local inflammation. Under stress， activin A levels are elevated， effecting inflammatory mediators or itself as a mediator of inflammation activated vascular endothelial cells stimulate E-selectin secretion and synthesis， induced inflammatory reaction and to cause the disorder of lipid metabolism in the body′s physiological balance， causing Shanghuo.
Key Words Shanghuo； Activin A； E selectin； Inflammatory reaction
中图分类号：R285.6文献标识码：Adoi：10.3969/j.issn.1673-7202.2017.12.005
“上火”是指人体阴阳失衡，表现为目赤、口疮、口腔潰疡、牙龈肿痛、咽痛、口干、尿赤、便干等一系列热象的疾病[1]。课题组前期大规模流行病学调查发现，引起“上火”的因素主要有情志、饮食、药物等[2]。“上火”常见实热“上火”与阴虚“上火”2种证型[3]。课题组前期利用细胞因子芯片检测6例阴虚“上火”患者“上火”前后血清细胞因子的变化，结果发现下调的细胞因子有10种，上调的细胞因子有23种，其中发现细胞因子Activin A和E-selectin明显升高，差异有统计学意义(P<0.05)，提示细胞因子在“上火”的发病过程中发挥作用。本研究拟在上述基础上，进一步从细胞分子生物学角度探讨Activin A和E-selectin对2种不同类型上火患者的影响。
1 资料与方法
1.1 一般资料 选取浙江中医药大学第一附属医院招募的临床志愿者48例，随机分为实热组、阴虚组、对照组，每组16例，其中男21例，女27例，平均年龄为38.83岁。分别采集促凝管及抗凝管血液，促凝管提取血清，抗凝管提取单核细胞，3组间年龄及性别比较，差异无统计学意义(P>0.05)。见表1。, http://www.100md.com(汪琴静 徐莉 包洁 季巾君 谢冠群 范永升)
参见：首页 > 中医药 > 中医药常识 > 中医基础 > 上火