摘要 目的：研究健脾安肠丸对腹泻型肠易激综合征(IBS-D)结肠组织SP、VIP的影响。方法：采用大黄灌胃与束缚配合的方法制备IBS-D大鼠。将60只成年SD大鼠随机分为4组：正常组、模型组、健脾安肠丸大剂量组、健脾安肠丸小剂量组和匹维溴铵组。采用ELISA法检测大鼠结肠组织中P物质(SP)、血管活性肠肽(VIP)和降钙素基因相关肽(CGRP)的含量。结果：模型组大鼠大便形状的评分值较正常组明显增高(P<0.01)，而健脾安肠丸大剂量组和匹维溴铵组较模型组分值显著下降(P<0.01，P<0.05)；大剂量、小剂量健脾安肠丸和匹维溴铵均能减少模型大鼠结肠SP、VIP含量，提高CGRP含量。结论：健脾安肠丸能有效地缓解腹泻型肠易激综合征，其疗效可能与减少SP、VIP和增加CGRP的释放有关。

    关键词 健脾安肠丸；腹泻型肠易激综合征；SP；VIP；CGRP

    Abstract Objective：To study the effects of Jianpi Anchang pill on SP and VIP in the colon tissue of Diarrhea-predominate Pattern Irritable Bowel Syndrome (IBS-D). Methods：The IBS-D rat model was established by gavage of Radix et Rhizoma Rhei and binding limbs. A total of 60 adult SD rats were randomly divided into 4 groups：normal group ......