摘要 目的：利用土培植物进行原位侵染获得RNAi型丹参毛状根，并对其干扰效果进行研究。方法：利用转化有目标基因RNAi载体的发根农杆菌对土壤中生长的丹参幼苗根茎部分进行侵染产生毛状根，经荧光显微镜鉴定阳性毛状根，并利用实时荧光定量PCR检测目标基因的表达情况。结果：在土培丹参根茎部分成功侵染产生毛状根，利用荧光显微镜进行检测显示目标基因RNAi型毛状根阳性转化率为72.72%，RNAi空载毛状根阳性转化率为66.67%。实时荧光定量PCR结果表明RNAi型毛状根中目标基因表达受到抑制，表达量下降到空载型对照组的8.61%，证明对土培植物直接进行侵染能够成功诱导RNAi毛状根。结论：采用原位侵染法诱导丹参根茎部位成功产生阳性RNAi型毛状根，侵染过程无需无菌操作，简单快捷，对植株生长无影响，能够用于功能基因的体内功能研究。

    关键词 丹参；毛状根；原位侵染；RNAi

    Abstract Objective：To study the jamming effects of RNAi hairy roots of Salvia Miltiorrhiza by in situ infection of soil cultivating plants.Methods：Hairy roots were induced by infection of Agrobacterium rhizogenes transformed with the RNAi vector of target gene into the roots of Salvia Miltiorrhiza which were planted in the soil.Positive hairy roots were identified by fluorescence microscope.The expression of target gene was detected by qRT-PCR analysis.Results：Hairy roots were induced from the roots of Salvia Miltiorrhiza successfully.GFP detection results showed that positive conversion rates of hairy root lines of target gene RNAi and empty vector were 72.72% and 66.7% ......