丹参酮ⅡA对大鼠急性脊髓损伤SOCS3/STAT3信号通路的影响(1)
摘要目的：通过观察丹参酮ⅡA对大鼠急性脊髓损伤后细胞因子信号抑制因子3(Suppressors of Cytokine Signaling3，SOCS3)/信号转导与转录激活子3(Signal Transducer and Activator of Transcription3，STAT3)信号通路表达的影响，探讨丹参酮ⅡA对大鼠急性脊髓损伤炎性反应的影响。方法：60只健康SD大鼠随机分为脊髓损伤组(对照组)、脊髓损伤/丹参酮IIA治疗组(观察组)2组，每组30只。制作脊髓损伤动物模型。各组分别在脊髓损伤后8 h、1 d、3 d、7 d、14 d取材。分析大鼠脊髓损伤后运动诱发电位(MEP)的潜伏期及波幅；RTPCR半定量分析脊髓组织中SOCS3 mRNA、STAT3 mRNA的表达；TUNEL法行神经细胞凋亡检测。结果：与对照组比较，观察组MEP潜伏期差异无统计学意义(P>005)，MEP波幅则明显增高(P<005)；SOCS3 mRNA表达明显增高、STAT3mRNA表达明显下降；神经细胞凋亡有所减少(P<005)。结论：丹参酮IIA可提高SOCS3表达，抑制STAT3表达，抑制神经细胞的凋亡，减轻神经功能缺失，对急性脊髓损伤大鼠有一定的保护作用。
, http://www.100md.com
关键词丹参酮ⅡA；急性脊髓损伤；细胞因子信号抑制因子3；信号转导与转录激活子3
Effects of Tanshinone ⅡA on SOCS3/STAT3 Signaling Pathway in Rats with Acute Spinal Cord Injury
Nie Yu1，Zhou Naxin1，Liu Jian1，Wan Feng1，Fu Dehao2
(1 Department of Orthopedics，Yichang Central Hospital，Yichang 443000，China； 2 Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology，Wuhan 430022，China)
, 百拇医药
AbstractObjective：To observe the effects of Tanshinone ⅡA on the expression of suppressors of cytokine signaling3，(SOCS3)/signal transducer and activator of transcription3 (STAT3) in rats with acute spinal cord injury.Methods：A total of 60 healthy adult SpragueDawley rats were randomly divided into two groups：control group and Tanshinone ⅡA group.The acute spinal cord injury model was built in rats.At 8 h，1 d，3 d，7 d，and 14 d after spinal cord injury，we analyzed latency and amplitude of MEP (Motion Evoked Potential)，and reverse transcription polymerase chain reaction (RTPCR) was used to detect the mRNA expressions of SOCS3 and STAT3 in spinal cord tissues.Apoptosis neurons were labeled with TUNEL dyeing.Results：There were no changes in the latencies of MEPs in both groups，and the mean amplitudes of MEPs were higher in Tanshinone ⅡA group than those in control group.The expressions of SOCS3 mRNA and STAT3 mRNA were increased in Tanshinone ⅡA group compared with control group.The number of TUNEL staining positive cells in the Tanshinone ⅡA group was less than that in control group (P<005).Conclusion：Tanshinone ⅡA may enhance the expression of SOCS3 and inhibit the expression of STAT3，inhibit neurocyte apoptosis and palliate loss of neural function.It has certain protective effects on rats with acute spinal cord injury.
Key WordsTanshinone ⅡA； Acute spinal cord injury； Suppressors of cytokine signaling3； Signal transducer and activator of transcription3
中圖分类号：R2855文献标识码：Adoi：10.3969/j.issn.1673-7202.2018.10.046, 百拇医药(聂宇 周纳新 刘健)
, http://www.100md.com
关键词丹参酮ⅡA；急性脊髓损伤；细胞因子信号抑制因子3；信号转导与转录激活子3
Effects of Tanshinone ⅡA on SOCS3/STAT3 Signaling Pathway in Rats with Acute Spinal Cord Injury
Nie Yu1，Zhou Naxin1，Liu Jian1，Wan Feng1，Fu Dehao2
(1 Department of Orthopedics，Yichang Central Hospital，Yichang 443000，China； 2 Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology，Wuhan 430022，China)
, 百拇医药
AbstractObjective：To observe the effects of Tanshinone ⅡA on the expression of suppressors of cytokine signaling3，(SOCS3)/signal transducer and activator of transcription3 (STAT3) in rats with acute spinal cord injury.Methods：A total of 60 healthy adult SpragueDawley rats were randomly divided into two groups：control group and Tanshinone ⅡA group.The acute spinal cord injury model was built in rats.At 8 h，1 d，3 d，7 d，and 14 d after spinal cord injury，we analyzed latency and amplitude of MEP (Motion Evoked Potential)，and reverse transcription polymerase chain reaction (RTPCR) was used to detect the mRNA expressions of SOCS3 and STAT3 in spinal cord tissues.Apoptosis neurons were labeled with TUNEL dyeing.Results：There were no changes in the latencies of MEPs in both groups，and the mean amplitudes of MEPs were higher in Tanshinone ⅡA group than those in control group.The expressions of SOCS3 mRNA and STAT3 mRNA were increased in Tanshinone ⅡA group compared with control group.The number of TUNEL staining positive cells in the Tanshinone ⅡA group was less than that in control group (P<005).Conclusion：Tanshinone ⅡA may enhance the expression of SOCS3 and inhibit the expression of STAT3，inhibit neurocyte apoptosis and palliate loss of neural function.It has certain protective effects on rats with acute spinal cord injury.
Key WordsTanshinone ⅡA； Acute spinal cord injury； Suppressors of cytokine signaling3； Signal transducer and activator of transcription3
中圖分类号：R2855文献标识码：Adoi：10.3969/j.issn.1673-7202.2018.10.046, 百拇医药(聂宇 周纳新 刘健)
参见：首页 > 医疗版 > 疾病专题 > 骨科 > 骨折 > 脊柱、脊髓损伤