前列宁胶囊调控MMP-2影响细胞外基质对良性前列腺增生治疗的影响(1)
摘要 目的：觀察前列宁胶囊(QC)对基质金属蛋白酶-2(MMP-2)介导的细胞外基质(ECM)的影响，探讨QC对BPH的治疗机制。方法：SD大鼠随机分为空白组，BPH模型组，QC低、中、高观察组，建立BPH模型，QC干预，ELISA法检测大鼠血清MMP-2、FN、Collagen IV、LN;BPH-1细胞培养，分别加入MMP-2和未加MMP-2刺激因子，QC干预，MTT法观察细胞活性，Q-PCR及Western-Blot分别检测MMP-2、FN、Collagen IV、LN基因及蛋白表达(未加MMP-2)，加入MMP-2组检测FN、Collagen IV、LN。结果：QC各剂量组大鼠血清中的FN、LN、Collagen IV含量降低，MMP-2升高( P <0.05 or P <0.01);BPH-1细胞培养QC干预，加入及未加入MMP-2刺激因子细胞活力均有下降，以48 h后最明显;加入MMP-2刺激因子FN、Collagen IV、LN基因及蛋白表达明显低于未加MMP-2刺激因子，差异有统计学意义。结论：QC对BPH具有治疗作用，QC调控MMP-2介导细胞外基质FN、Collagen IV、LN基因和蛋白表达可能是其治疗BPH机制之一。
, http://www.100md.com
关键词 前列腺增生;前列宁胶囊;ECM;MMP-2;FN;Collagen IV;LN;Q-PCR;Western-Blot
Effects of Qianliening Capsules in Regulating Extracellular Matrix via MMP-2 on Treating Benign Prostatic Hyperplasia
Zhou Jianheng 1，Yang Hong 2，Lin Jiumao 2，Hong Zhenfeng 1
(1 Department of Integrative Medicine of Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China; 2 Fujian Academy of Integrative Medicine of Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China)
, 百拇医药
Abstract Objective： To observe the effects of Qianliening Capsules (QC) on the extracellular matrix (ECM) via MMP-2 in treating benign prostatic hyperplasia (BPH)，and to explore the therapeutic mechanism of QC on BPH. Methods： Sprague-Dawley rats were randomly divided into blank group，BPH model group，QC low，middle，and high treatment groups.BPH rat model was established and intervened by QC.The MMP-2，LN，FN，Collagen IV in serum were examined by ELISA.BPH-1 cells without MMP-2 stimulators and BPH-1 cells added with MMP-2 stimulators were cultured respectively and treated by QC.The cell morphology and viability were observed by light microscope and MTT.The mRNA and protein expression of MMP-2，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (no MMP-2)，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (MMP-2 was added). Results： The levels of Collagen IV，FN，LN in serum of BPH rats serum treated with QC groups were reduced significantly and MMP-2 was significantly increased (P﹤0.05 or P﹤0.01).The mRNA and protein expression of LN，FN，Collagen IV in BPH-1 cells QC-treated groups were decreased significantly，and the result was more obvious in the MMP-2 added groups，but the MMP-2 was enhanced in BPH-1 cells QC-treated groups，with significant difference. Conclusion： QC has significant therapeutic effects on BPH.QC can inhibit the expression of FN，Collagen IV and LN，and enhance MMP-2，and MMP-2 can regulate FN，Collagen IV and LN，QC regulate the expression of ECM via MMP-2 is one of the mechanisms by which QC treats BPH., http://www.100md.com(周建衡 杨弘 林久茂 洪振丰)
, http://www.100md.com
关键词 前列腺增生;前列宁胶囊;ECM;MMP-2;FN;Collagen IV;LN;Q-PCR;Western-Blot
Effects of Qianliening Capsules in Regulating Extracellular Matrix via MMP-2 on Treating Benign Prostatic Hyperplasia
Zhou Jianheng 1，Yang Hong 2，Lin Jiumao 2，Hong Zhenfeng 1
(1 Department of Integrative Medicine of Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China; 2 Fujian Academy of Integrative Medicine of Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China)
, 百拇医药
Abstract Objective： To observe the effects of Qianliening Capsules (QC) on the extracellular matrix (ECM) via MMP-2 in treating benign prostatic hyperplasia (BPH)，and to explore the therapeutic mechanism of QC on BPH. Methods： Sprague-Dawley rats were randomly divided into blank group，BPH model group，QC low，middle，and high treatment groups.BPH rat model was established and intervened by QC.The MMP-2，LN，FN，Collagen IV in serum were examined by ELISA.BPH-1 cells without MMP-2 stimulators and BPH-1 cells added with MMP-2 stimulators were cultured respectively and treated by QC.The cell morphology and viability were observed by light microscope and MTT.The mRNA and protein expression of MMP-2，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (no MMP-2)，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (MMP-2 was added). Results： The levels of Collagen IV，FN，LN in serum of BPH rats serum treated with QC groups were reduced significantly and MMP-2 was significantly increased (P﹤0.05 or P﹤0.01).The mRNA and protein expression of LN，FN，Collagen IV in BPH-1 cells QC-treated groups were decreased significantly，and the result was more obvious in the MMP-2 added groups，but the MMP-2 was enhanced in BPH-1 cells QC-treated groups，with significant difference. Conclusion： QC has significant therapeutic effects on BPH.QC can inhibit the expression of FN，Collagen IV and LN，and enhance MMP-2，and MMP-2 can regulate FN，Collagen IV and LN，QC regulate the expression of ECM via MMP-2 is one of the mechanisms by which QC treats BPH., http://www.100md.com(周建衡 杨弘 林久茂 洪振丰)