紫草素对银屑病T淋巴细胞增殖、活化的影响(1)
摘要 目的：研究具有涼血、解毒作用的中药单体紫草素对银屑病病理状态下异常活化的T淋巴细胞功能及相关信号转导通路的影响。方法：选取Jurkat E6-1 T淋巴细胞，以佛波醇酯和离子霉素活化Jurkat E6-1 T淋巴细胞，同时将0.5～2 μg/mL浓度的紫草素作用于细胞，采用CCK-8法检测T淋巴细胞增殖能力;流式细胞术检测细胞膜CD69的表达及细胞内游离钙离子浓度([Ca 2+]i)的变化;用酶联免疫吸附试验(ELISA)检测T淋巴细胞释放白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)的水平;用Realtime-PCR和Western blotting法分别观察核转录因子mRNA及蛋白的表达影响。结果：不同浓度的紫草素均可明显抑制细胞增殖、CD69表达及Th1类细胞因子分泌;均可有效降低[Ca 2+]i和蛋白激酶C(PKC)磷酸化蛋白的水平;显著降低核转录因子NF-AT mRNA的表达，下调c-Jun的mRNA及蛋白表达并可抑制核因子-κB蛋白的表达。以上各项指标均有一定量效关系。结论：紫草素可以通过抑制过度活化的T淋巴细胞功能从而发挥免疫调控作用，为紫草素治疗银屑病的作用机制及应用前景提供实验依据。
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关键词 紫草素;T淋巴细胞活化;信号转导通路;银屑病;凉血解毒
Effects of Shikonin on Proliferation and Activation of T Lymphocytes in Psoriasis
Liu Xin，Zhao Jingxia，Wang Yan，Li Ping
(1 Beijing Hospital of Traditional Chinese Medicine，Capital Medical University/Beijing Institute of Traditional Chinese Medicine，Beijing 100010，China)
Abstract Objective： To study the effects of shikonin，which is a kind of monomer of Chinese traditional herbs with cooling blood and detoxification，on the function of abnormally activated T lymphocytes and related signal transduction pathways in the pathological state of psoriasis in vitro cell model. Methods： Jurkat E6-1 T lymphocytes were activated with phorbol ester and ionomycin，and shikonin at a concentration of 0.5-2 μg/mL was applied to the cells.T lymphocyte proliferation ability was detected by CCK-8 method.The flow cytometry was used to detect the expression of CD69 and the change of intracellular free calcium concentration ([Ca 2+]i).The enzyme-linked immunosorbent assay (ELISA) was used to detect the release of interleukin-2 (IL-2)，interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α); q-PCR and Western blot were used to observe the effects of nuclear transcription factor mRNA and protein expression. Results： Different concentrations of shikonin can significantly inhibit cell proliferation，CD69 expression and Th1 cytokine secretion; both can effectively reduce the content of [Ca 2+]i and protein kinase C (PKC) phosphorylated protein，significantly reduce nuclear transcription factor NF-The expression of AT mRNA down-regulates the mRNA and protein expression of c-Jun and inhibit the expression of NF-κB protein.All the above indicators had a certain dose-effect relationship. Conclusion： Shikonin can exert immunoregulatory effects by inhibiting the function of over-activated T lymphocytes，and provide experimental evidence for the mechanism and application prospect of shikonin in the treatment of psoriasis., 百拇医药(刘欣 赵京霞 王燕 李萍)
, http://www.100md.com
关键词 紫草素;T淋巴细胞活化;信号转导通路;银屑病;凉血解毒
Effects of Shikonin on Proliferation and Activation of T Lymphocytes in Psoriasis
Liu Xin，Zhao Jingxia，Wang Yan，Li Ping
(1 Beijing Hospital of Traditional Chinese Medicine，Capital Medical University/Beijing Institute of Traditional Chinese Medicine，Beijing 100010，China)
Abstract Objective： To study the effects of shikonin，which is a kind of monomer of Chinese traditional herbs with cooling blood and detoxification，on the function of abnormally activated T lymphocytes and related signal transduction pathways in the pathological state of psoriasis in vitro cell model. Methods： Jurkat E6-1 T lymphocytes were activated with phorbol ester and ionomycin，and shikonin at a concentration of 0.5-2 μg/mL was applied to the cells.T lymphocyte proliferation ability was detected by CCK-8 method.The flow cytometry was used to detect the expression of CD69 and the change of intracellular free calcium concentration ([Ca 2+]i).The enzyme-linked immunosorbent assay (ELISA) was used to detect the release of interleukin-2 (IL-2)，interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α); q-PCR and Western blot were used to observe the effects of nuclear transcription factor mRNA and protein expression. Results： Different concentrations of shikonin can significantly inhibit cell proliferation，CD69 expression and Th1 cytokine secretion; both can effectively reduce the content of [Ca 2+]i and protein kinase C (PKC) phosphorylated protein，significantly reduce nuclear transcription factor NF-The expression of AT mRNA down-regulates the mRNA and protein expression of c-Jun and inhibit the expression of NF-κB protein.All the above indicators had a certain dose-effect relationship. Conclusion： Shikonin can exert immunoregulatory effects by inhibiting the function of over-activated T lymphocytes，and provide experimental evidence for the mechanism and application prospect of shikonin in the treatment of psoriasis., 百拇医药(刘欣 赵京霞 王燕 李萍)
参见：首页 > 医疗版 > 疾病专题 > 皮肤科 > 丘疹鳞屑性皮肤病 > 银屑病(牛皮癣)