摘要 目的：建立门氏柴胡理中颗粒定性定量质量标准控制方法。方法：采用薄层色谱法对门氏柴胡理中颗粒制剂中柴胡、黄芩进行薄层鉴别，采用高效液相色谱法(HPLC)对柴胡理中颗粒中的黄芩苷进行含量测定，色谱柱为Diamonsil-C18色谱柱(200 mm×4.6 mm，5.0 μm)，流动相为甲醇-0.1%醋酸水溶液进行梯度洗脱，流速为1.0 mL/min，检测波长为280 nm，柱温为25 ℃，进样量为10 μL.结果：柴胡、黄芩薄层色谱斑点分离清晰，阴性均无干扰;回归方程为y=2 907 186.561 0x+21 224.436 6(r=0.999 8)，黄芩苷在0.12～1.20 μg范围内具有良好的线性关系。平均加样回收率为99.45%，RSD为2.48%。结论：建立的方法方便、可靠，可作为门氏柴胡理中颗粒的薄层鉴别和含量测定质量控制方法。

    关键词 门氏柴胡理中颗粒;薄层色谱;高效液相色谱法;含量测定;柴胡;黄芩;半夏;黄芩苷

    Abstract Objective：To establish a qualitative and quantitative quality standard control method for the Menshi Chaihu Lizhong Granules.Methods：The Bupleurum chinense and Radix Astragali were qualitatively identified by thin-layer chromatography by the Menshi Chaihu Lizhong Granules.The content of baicalin in Menshi Chaihu Lizhong was determined by high performance liquid chromatography.Diamonsil-C18 column(200 mm×4.6 mm ......