三七总皂苷对脊髓损伤大鼠神经元凋亡及Nrf2、caspase-3表达的影响(1)
摘要 目的：探究三七总皂苷(TSPN)对脊髓损伤(SCI)大鼠神经元凋亡及Nrf2、Caspase-3表达的影响。方法：采用“自制击打器”建立SCI大鼠模型，将成功构建的100只SCI大鼠随机分为对照组、模型组、阳性对照组及实验组，每组25只。阳性对照组与实验组分别腹腔注射甲基强的松龙[30 mg/(kg·d)]与TSPN[100 mg/(kg·d)]，对照组与模型组则腹腔注射等量生理盐水，连续干预14 d。采用HE染色与TUNEL法检测大鼠脊髓组织病理学变化与神经元凋亡;采用免疫组化法及Wb法检测脊髓组织Nrf2、Caspase-3蛋白表达。结果：与对照组比较，模型组大鼠脊髓组织Nrf2蛋白表达显著降低，且阳性对照组与实验组高于模型组，阳性对照组高于实验组;神经元凋亡率及Caspase-3蛋白表达升高，且阳性对照组与实验组低于模型组，阳性对照组低于实验组(均P<0.05)。结论：TSPN可减轻SCI大鼠脊髓组织病变程度，保护神经元，其作用机制可能与下调脊髓组织Caspase-3蛋白表达，上调Nrf2蛋白表达有关。
关键词 脊髓损伤;三七总皂苷;神经元凋亡;Nrf2;Caspase-3
Abstract Objective：To explore the effects of Panax notoginseng saponins(TSPN)on neuronal apoptosis and expression of Nrf2 and Caspase-3 in rats with spinal cord injury(SCI).Methods：Rat models were established with the “self-made beater” and 100 successfully constructed SCI rats were randomly divided into a control group，a model group，a positive control group，and a test group，with 25 rats in each group.Positive control group and the test group were given methylprednisolone[30 mg/(kg·d)]and TSPN[100 mg/(kg·d)]by intraperitoneal injection respectively; the control group and the model group were given the same amount of normal saline for 14 d.HE staining and TUNEL method were used to detect the histopathological changes and neuronal apoptosis of rat spinal cord tissue; the protein expression of Nrf2 and Caspase-3 in the spinal cord tissues were detected by immunohistochemistry and Wb method.Results：Compared with the control group，the expression of Nrf2 protein in the spinal cord tissues of rats in the model group significantly decreased，and the positive control group and the test group were higher than the model group，and the positive control group was higher than the test group; and the neuronal apoptosis rate and Caspase-3 protein expression significantly increased，and the positive control group and the test group were lower than the model group，and the positive control group was lower than the test group(all P<0.05).Conclusion：TSPN can alleviate the degree of spinal cord tissue lesions in SCI rats and protect neurons，and the mechanisms may be related to down-regulation of Caspase-3 protein expression and up-regulation of Nrf2 protein expression in spinal cord tissues.
Keywords Spinal cord injury; Panax notoginseng saponins; Neuronal apoptosis; Nrf2; Caspase-3
脊髓损伤(Spinal Cord Injury，SCI)包括原发性和继发性损伤，具有较高的致残率，同时由于目前对其发病机制尚未完全了解，所以仍缺乏有效治療药物[1]。三七总皂苷(TSPN)是三七的主要活性成分，具有抗炎、抗菌及抗氧化等多种药理功效，近年来，其在神经保护方面的研究成为热点。研究发现，TSPN可促进全脑缺血后大鼠脑室下区(SVZ)神经再生，加速SVZ区神经祖细胞增殖和分化，促进星形胶质细胞转化新生未成熟神经元[2]。也有研究报道，黄芪甲苷和TSPN配伍可抑制小鼠脑缺血再灌注后神经细胞的凋亡，发挥保护神经细胞的作用[3]。目前TSPN在SCI方向的研究尚少，因此开展本研究。, http://www.100md.com(谢慕可 许念茹 黄贤胜)
关键词 脊髓损伤;三七总皂苷;神经元凋亡;Nrf2;Caspase-3
Abstract Objective：To explore the effects of Panax notoginseng saponins(TSPN)on neuronal apoptosis and expression of Nrf2 and Caspase-3 in rats with spinal cord injury(SCI).Methods：Rat models were established with the “self-made beater” and 100 successfully constructed SCI rats were randomly divided into a control group，a model group，a positive control group，and a test group，with 25 rats in each group.Positive control group and the test group were given methylprednisolone[30 mg/(kg·d)]and TSPN[100 mg/(kg·d)]by intraperitoneal injection respectively; the control group and the model group were given the same amount of normal saline for 14 d.HE staining and TUNEL method were used to detect the histopathological changes and neuronal apoptosis of rat spinal cord tissue; the protein expression of Nrf2 and Caspase-3 in the spinal cord tissues were detected by immunohistochemistry and Wb method.Results：Compared with the control group，the expression of Nrf2 protein in the spinal cord tissues of rats in the model group significantly decreased，and the positive control group and the test group were higher than the model group，and the positive control group was higher than the test group; and the neuronal apoptosis rate and Caspase-3 protein expression significantly increased，and the positive control group and the test group were lower than the model group，and the positive control group was lower than the test group(all P<0.05).Conclusion：TSPN can alleviate the degree of spinal cord tissue lesions in SCI rats and protect neurons，and the mechanisms may be related to down-regulation of Caspase-3 protein expression and up-regulation of Nrf2 protein expression in spinal cord tissues.
Keywords Spinal cord injury; Panax notoginseng saponins; Neuronal apoptosis; Nrf2; Caspase-3
脊髓损伤(Spinal Cord Injury，SCI)包括原发性和继发性损伤，具有较高的致残率，同时由于目前对其发病机制尚未完全了解，所以仍缺乏有效治療药物[1]。三七总皂苷(TSPN)是三七的主要活性成分，具有抗炎、抗菌及抗氧化等多种药理功效，近年来，其在神经保护方面的研究成为热点。研究发现，TSPN可促进全脑缺血后大鼠脑室下区(SVZ)神经再生，加速SVZ区神经祖细胞增殖和分化，促进星形胶质细胞转化新生未成熟神经元[2]。也有研究报道，黄芪甲苷和TSPN配伍可抑制小鼠脑缺血再灌注后神经细胞的凋亡，发挥保护神经细胞的作用[3]。目前TSPN在SCI方向的研究尚少，因此开展本研究。, http://www.100md.com(谢慕可 许念茹 黄贤胜)
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