王海燕++++++涂林修++++++黄艳丽++++++陈凯宇++++++冯碧红

    [摘要] 目的 探讨宁波地区儿童哮喘与ORMDL3基因多态性的关系。 方法 采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术对150例哮喘患儿(哮喘组)ORMDL3基因多态性进行观察，并与150名正常对照儿童(对照组)进行对比。 结果 哮喘组和对照组ORMDL3基因rs11650680位点3种基因型(CC、CT、TT)分布频率分别为68%、24%、8%和57%、25%、18%，差异有统计学意义(χ2=6.574，P=0.037)，此位点C和T等位基因分布频率分别为80%、20%和70%、30%，差异有统计学意义(χ2=8.000，P=0.005)；哮喘组和对照组ORMDL3基因rs4378650位点各基因型(CC、CT、TT)频率分别为59%、27%、14%和53%、21%、26%，差异有统计学意义(χ2=7.654，P=0.020)，此位点C和T等位基因分布频率分别为73%、27%和63%、37%，差异有统计学意义(χ2=6.424，P=0.010)。 结论 ORMDL3基因多态性与宁波地区儿童哮喘患者有一定的相关性。

    [关键词] 基因多态性；儿童哮喘；ORMDL3

    [中图分类号] R725[文献标识码] A[文章编号] 1673-7210(2014)06(c)-0027-04

    Analysis of relationship between ORMDL3 gene polymorphism with childhood asthma in Ningbo

    WANG Haiyan TU Linxiu HUANG Yanli CHEN Kaiyu FENG Bihong

    Department of Pediatrics, the People's Hospital of Beilun District in Ningbo City, Zhejiang Province, Ningbo 315800, China

    [Abstract] Objective To investigate the relationship between ORMDL3 gene polymorphism and childhood asthma in Ningbo. Methods Polymorphism of ORMDL3 gene was identified with polymerase chain reaction-restriction fragmented length polymorphism (PCR-RFLP) in 150 childhood asthma patients (asthma group) and 150 healthy controls (control group), the results were compared. Results The distribution frequency of ORMDL3 gene rs11650680 locus genotypes including CC, CT and TT in the asthma group and control groups were 68%, 24%, 8% and 57%, 25%, 18%, respectively, there was a significant difference between the asthma group and control groups (χ2 = 6.574, P = 0.037). The locus C and T allele frequencies were 80%, 20% and 70%, 30%, respectively, and there was a significant difference between asthma group and control group (χ2 = 8.000, P = 0.005). The distribution frequency of ORMDL3 gene rs4378650 locus genotypes including CC, CT and TT were 59%, 27%, 14% and 53%, 21%, 26%, respectively, and the difference was statistically significant (χ2 = 7.654, P = 0.020). The locus C and T allele frequencies were 73%, 27% and 63%, 37%, respectively, there was a significant difference between the asthma group and control group (χ2 = 6.424 ......