叶婷+宁勇

    [摘要] 目的 探讨过氧化物酶增殖物激活受体-γ(PPARγ)和核因子E2相关因子-2(Nrf2)在肾脏衰老中的作用。方法 以3个月龄和24个月龄大鼠作为青年组和衰老组(n = 10)，PAS染色观察肾脏组织病理改变，肾脏组织匀浆测定H2O2、丙二醛(MDA)含量，Western Blot法检测肾脏组织髓过氧化物酶(MPO)、PPARγ和Nrf2蛋白质表达。 结果 衰老组大鼠肾脏组织MDA[(44.42±4.17)μmol/mg比(26.23±1.43)μmol/mg]、H2O2[(239.90±39.38) μmol/μg比(110.00±19.49)μmol/μg]含量较青年组均明显增加，差异有统计学意义(P 0.05)。 结论 氧化损伤在肾脏衰老中发挥重要作用，可能是通过PPARγ表达下降介导。

    [关键词] 肾脏衰老；氧化应激；过氧化物酶增殖物激活受体-γ；核因子E2相关因子-2

    [中图分类号] R3 [文献标识码] A [文章编号] 1673-7210(2014)10(c)-0004-04

    Role of peroxisome proliferator-activated receptor–γ and nuclear factor-E2-related factor-2 on kidney aging

    YE Ting1 NING Yong2

    1.Department of Clinic Nutrition， Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology， Hubei Province， Wuhan 430030， China； 2.Department of Nephrology， Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology， Hubei Province， Wuhan 430030， China

    [Abstract] Objective To observe the role of peroxisome proliferator-activated receptor gamma (PPARγ) and nuclear factor- E2- related factor-2 (Nrf2) on the kidney aging. Methods Rat aging model was established using 3- month and 24-month old rats as young group and aging group (n = 10). Renal pathological changes were tested by PAS reaction. The amount of the H2O2 and MDA were tested using kidney homogenate. Western blotting was used to determine the protein expression of MPO ......