滕娜等

    [摘要] 目的 探讨吸入氢气(H2)对脓毒血症大鼠急性肺损伤的影响。 方法 选取雄性SD大鼠40只，体重200～250 g。采用随机数字表法将其分为4组(n = 10)：假手术组(SH组)、盲肠结扎穿孔组(CLP组)、1% H2组和2% H2组。采用CLP法制备脓毒血症模型。1% H2组与2% H2组于CLP术后12 h分别吸入1% H2与2% H2 2 h。结束后，采集股动脉血样行血气分析，计算氧合指数(PaO2/FiO2)；测定血浆中高迁移率族蛋白1(HMGB1)、白细胞介素-6(IL-6)水平；随后处死大鼠取肺组织，检测肺组织中Toll样受体4(TLR-4)、核因子-кB(NF-κB)、髓过氧化物酶(MPO)活性；光镜下观察肺组织病理改变。 结果 与SH组比较，CLP组、1% H2组、2% H2组PaO2/FiO2降低，血浆中HMGB1、IL-6水平升高，肺组织TLR-4、NF-κB、MPO水平升高，病理学损伤明显加重(P 0.05)。 结论 吸入H2能够减轻脓毒血症引起的急性肺损伤，其机制可能与抑制炎性反应有关。

    [关键词] 氢气；脓毒血症；大鼠；肺损伤

    [中图分类号] R631 [文献标识码] A [文章编号] 1673-7210(2015)09(a)-0008-03

    [Abstract] Objective To investigate the effect of hydrogen (H2) inhalation on acute lung injury in rats with sepsis. Methods Forty healthy male SD rats (weighing 200-250 g) were randomly divided into 4 groups (n = 10) using a random number table： sham operation group (SH group)， cecal ligation and puncture group (CLP group)， 1% H2 group， 2% H2 group respectively. Sepsis model was produced by CLP. 1% H2 and 2% H2 group received 2 h inhalation of 1% H2 and 2% H2 at 12 h after CLP ......