石柳柳 李贺然苏州大学医学部药学：，江苏苏州215123

    黑老虎中倍半萜类化合物的分离鉴定及抑制NO生成作用研究

    石柳柳李贺然

    苏州大学医学部药学：，江苏苏州215123

    目的分离鉴定黑老虎中倍半萜类化合物，并考察化合物对一氧化氮(NO)释放的抑制作用。方法采用丙酮提取，半制备高效液相色谱等方法分离化学成分，波谱方法鉴定化合物的结构；脂多糖(LPS)和γ干扰素(IFN-γ)诱导的单核巨噬细胞RAW264.7作为细胞炎症模型，考察化合物对NO释放的抑制作用。结果分离得到了4个倍半萜类化合物，经波谱方法鉴定，分别为匙叶桉油烯醇(1)、(-)-7βH-柳杉二醇(2)、(-)-依兰油醇(3)、(+)-顺-菖蒲烯(4)。化合物1较强抑制LPS/INF-γ诱导NO的产生。结论化合物1～4均首次从南五味子属中分离得到，化合物1具有抗炎活性。

    黑老虎；倍半萜；NO；抑制作用

    [Abstract]Objective To study the sesquiterpenes from kadsura coccinea and their nitric oxide(NO)inhibitory activities.Methods Acetone was used to extract,the compounds were iso1ated by semi-preparative HPLC method,their structures were identified by spectroscopy.The activated RAW 264.7 macrophages induced by LPS and IFN-γ were used to eva1uate the inhibitory activities on NO production.Results Four sesquiterpenes were iso1ated and identified as ent-spathu1eno1,(-)-7βH-eudesmane-4α，11-dio1,(-)-T-muuro1o1,(+)-cis-Ca1amenene.Compound 1 had strong inhibitory activity on NO production.Conclusion Compound 1-4 are obtained from the Kadsura for the first time. Compound 1 has the anti-inf1ammatory effect. ......