谭宏伟 楚光华 胡春艳 张恩娣西北妇女儿童医院妇科，陕西西安 710061

    三七总皂苷对子宫内膜癌Ishikawa和HEC-1A细胞增殖、侵袭和凋亡的影响

    谭宏伟楚光华胡春艳张恩娣

    西北妇女儿童医院妇科，陕西西安710061

    目的 探讨三七总皂苷(PNS)对子宫内膜癌Ishikawa和HEC-1A细胞增殖、侵袭和凋亡的影响及其作用机制。方法 应用不同浓度的PNS分别作用于Ishikawa和HEC-1A细胞24、48、72 h，采用MTT法检测细胞增殖率；Transwell检测细胞侵袭能力；流式细胞仪检测细胞凋亡水平；采用RT-PCR和Western blot分别检测VEGF、PI3K、AKT的mRNA和蛋白表达水平。 结果PNS可以显著抑制Ishikawa和HEC-1A细胞的增殖。与control组比较，PNS处理的Ishikawa和HEC-1A细胞侵袭显著下降(P<0.05)，细胞凋亡率明显升高(P<0.05)，VEGF和PI3K的表达水平下降(P<0.05)。结论PNS能够抑制Ishikawa和HEC-1A细胞的增殖和侵袭，诱导细胞凋亡，其过程可能与VEGF/PI3K/AKT信号通路的抑制有关。

    三七总皂苷；子宫内膜癌；血管内皮生长因子；PI3K/AKT信号通路

    [Abstract]Objective To investigate the effects of Panaxnotoginsengsaponins(PNS)on the cell proliferation，invasion and apoptosis in endometrial cancercell line Ishikawa and HEC-1A and its underlying mechanism.Methods Ishikawa and HEC-1A cells were treated with different density of PNS for 24，48，72 h.The MTT method was used to detect the cell proliferation rate after Ishikawa and HEC-1A；cell invasion ability was detected by Transwell method；cell apoptosis was measured by flow cytometry；the mRNA and protein expression level of VEGF ......