何启艇 邱波 龚明

    [摘要] 目的 比较壳聚糖薄膜培养和常规平板培养对间充质干细胞(MSCs)干性相关基因表达的影响。 方法 从脐带中分离MSCs并分别进行壳聚糖薄膜培养和常规平板培养。采用Real-time RT-PCR和Western blot方法检测MSCs干性相关基因Nanog、Sox2、c-Myc、Oct-4 mRNA及其蛋白的表达。 结果 壳聚糖薄膜培养的MSCs能形成类球状体。壳聚糖薄膜培养的MSCs干性相关基因Nanog、Sox2、c-Myc、Oct-4 mRNA及其蛋白的表达水平较常规培养组显著增加，差异有高度统计学意义(P < 0.01)。 结论 与常规培养的MSCs比较，壳聚糖薄膜培养的MSCs能形成类球状体，更能保持MSCs的干性。

    [关键词] 壳聚糖；间充质干细胞；干性；骨关节炎

    [中图分类号] R329.2 [文献标识码] A [文章编号] 1674-4721(2016)10(b)-0004-04

    [Abstract] Objective To investigate the effects of cultivation modes (chitosan membrane and conventional plate) on the stemness of mesenchymal stem cells (MSCs) by comparing the related genes expression. Methods MSCs were isolated from umbilical cords and cultured on chitosan membrane and conventional plate. mRNA expression concerned with stemness related genes including Nanog， Sox2， c-Myc and Oct-4 were assayed by real-time reverse transcriptase polymerase chain reaction. Corresponding proteins were detected by Western blot. Results Spheroid was formed by MSCs cultured on chitosan membrane. Compared with MSCs cultured on conventional plate， mRNA and protein expression levels of Nanog ......