乌梅喷雾剂对大鼠颌下腺放射性损伤的影响
陈倩怡 李碧霞 叶景云[摘要] 目的 探討乌梅喷雾剂对大鼠颌下腺放射性损伤的影响。 方法 健康SPF级雄性6~7周Wistar大鼠30只采用随机数字表法分为正常组,模型组和高、中、低乌梅喷雾剂组,每组各6只。电子直线加速器一次性18 Gy局部照射建立放射性颌下腺细胞损伤模型。于用药后第14天收集唾液,计算唾液流率,检测唾液生化指标。处死后摘取颌下腺组织苏木精-伊红染色(HE染色)镜下观察颌下腺腺体组织形态学变化。 结果 用药后第14天,模型组及低、中、高浓度乌梅喷雾组大鼠体重均低于正常组,差异有统计学意义(P 0.05)。正常组大鼠腺体细胞排列规则,未见明显病理改变;模型组发生明显炎症细胞浸润,间质中胶原纤维明显增生,发生纤维化;各放射组大鼠颌下腺细胞均出现放射性损伤,与模型组比较,高浓度乌梅喷雾剂组颌下腺腺小叶细胞萎缩程度降低,腺体周围炎症细胞浸润减少,胞浆分泌物增多,但未能恢复至正常组水平。 结论 高浓度乌梅喷雾剂可一定程度上促进大鼠颌下腺放射性损伤细胞病理形态修复,但仍需进一步探讨相关机制。
[关键词] 乌梅喷雾剂;颌下腺;放射性损伤;大鼠
[中图分类号] R782 [文献标识码] A [文章编号] 1673-7210(2017)09(c)-0008-04
[Abstract] Objective To observe the influence of Dark Plum Sprays on radiation-induced submandibular gland damage of rats. Methods According to the random number table, a total of 30 healthy Wistar male rats aged 6-7 weeks were divided into normal group (NG), untreated group (UG), high-dose Dark Plum Sprays group (HG), medium-dose Dark Plum Sprays group (MG), low-dose Dark Plum Sprays group (LG), each group had 6 rats. The rats were given one-time 18 Gy irradiation locally on the submandibular glands. 14 days later, saliva was collected to calculate the salivary flow rate and detected the biochemical indexes. After that ......
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