陈琪 闵晶晶 王霄一

    [摘要] 目的 探討MiR-30c-2-3p对足细胞活力及Nephrin和Podocin蛋白的表达影响。 方法 采用嘌呤霉素(PAN)诱导的足细胞体外模型，将足细胞分成4组，即空白组、PAN组、阴性质粒+PAN组、阳性质粒+PAN组。通过LipofectamineTM 2000瞬时转染MiR-30c-2-3p mimic。通过Cell Counting Kit-8(CCK-8)法检测细胞活力、实时荧光定量PCR(qRT-PCR)检测细胞中MiR-30c-2-3p表达、Western blot检测Nephrin和Podocin蛋白的表达。 结果 ①CCK-8结果提示PAN组足细胞活力明显低于空白组，差异有高度统计学意义(P 0.05)，阳性质粒+PAN组足细胞活力与PAN组和阴性质粒+PAN组相比均有所提高，差异有统计学意义(P 0.05)，而阳性质粒+PAN组MiR-30c-2-3p的表达较PAN组和阴性质粒+PAN组均提高，差异有统计学意义(P 0.05)，而阳性质粒+PAN组Nephrin和Podocin蛋白的表达较单纯PAN组和阴性质粒+PAN组均增加，差异有统计学意义(P 0.05). The viability of positive plasmid+PAN group was significantly increased than that of the PAN group and the negative plasmid+PAN group， the differences were statistically significant (P 0.05). The content of MiR-30c-2-3p in the positive plasmid+PAN group was significantly higher than that of the PAN group and the negative plasmid+PAN group， the differences were statistically significant (P 0.05). Compared with the PAN group and the negative plasmid+PAN group ......