杨宪鲁 王海燕 汤华晓 丛涛 李玲芳

    [摘要] 目的 研究结节性红斑患者与健康对照人群外周血单核细胞中Toll样受体9(TLR9)和核因子-κB(NF-κB)的表达情况，并分析其在结节性红斑中的临床意义。 方法 选择2014年6月～2017年10月山东省威海市妇幼保健院及山东省威海市立医院确诊的结节性红斑患者52例作为疾病组；另选健康体检者41名作为对照组。采用实时荧光定量聚合酶链反应检测外周血单核细胞中TLR9和NF-κB的mRNA水平。 结果 疾病组外周血中TLR9 mRNA水平较对照组明显降低，差异有高度统计学意义(P < 0.01)，疾病组NF-κB mRNA水平较对照组明显升高，差异有高度统计学意义(P < 0.01)。疾病组中TLR9与NF-κB的mRNA水平存在线性正相关关系(r = 0.89，P < 0.01)。 结论 与正常人群相比，结节性红斑患者TLR9 mRNA水平明显降低，NF-κB mRNA水平明显升高，提示TLR9和NF-κB可能参与了结节性红斑疾病的发病过程。

    [关键词] 结节性红斑；外周血；Toll样受体9；核因子-κB

    [中图分类号] R275.986.1 [文献标识码] A [文章编号] 1673-7210(2018)09(b)-0106-03

    [Abstract] Objective To study the expression of Toll-like receptor 9 (TLR9) and nuclear factor-κB (NF-κB) in peripheral blood mononuclear cell of erythema nodosum patients and healthy controls and to explore the clinical significance of TLR9 and NF-κB in erythema nodosum. Methods From June 2014 to October 2017， 52 patients with erythema nodosum from Weihai Women and Children′s Hospital and Weihai Municipal Hospital of Shandong Province were enrolled as disease group， and 41 healthy physical examinees served as control group. The mRNA levels of TLR9 and NF-κB in peripheral blood mononuclear cell were detected by real-time fluorescence quantitative PCR technique. Results The level of TLR9 in disease group was lower than that of control group ......