李源 夏中元

    [摘要] 目的 探讨抗氧化剂N-乙酰半胱氨酸(NAC)对高脂高糖联合缺氧/复氧所致H9C2心肌细胞损伤及其对时钟基因BMAL1表达的影响。 方法 将培养的H9C2细胞分为对照组(N组)、高脂高糖联合缺氧/复氧组(HFHG+H/R组)、高脂高糖联合缺氧/复氧+NAC组(HFHG+H/R+NAC组)。CCK-8法检测细胞活性，流式细胞术检测细胞凋亡，DCFH-DA染色观察活性氧(ROS)水平，JC-1染色检测细胞线粒体膜电位(MMP)，Western blot测定BMAL1表达水平。 结果 与N组比较，HFHG+H/R组细胞活性明显降低(P < 0.01)，凋亡率与ROS水平明显升高(P < 0.01)，而BMAL1表达水平显著降低(P < 0.01)。经NAC处理后，HFHG+H/R+NAC组以上变化均显著减小，差异有高度统计学意义(P < 0.01)。HFHG+H/R组MMP较N组明显降低;经NAC处理后，HFHG+H/R+NAC组MMP明显升高。 结论 NAC可能通过抑制心肌细胞氧化应激水平，并促进时钟基因BMAL1表达，从而减小高脂高糖与缺氧/复氧所导致的心肌细胞损伤。

    [关键词] N-乙酰半胱氨酸;BMAL1;氧化应激;心肌细胞

    [中图分类号] R587.1? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-7210(2019)02(c)-0004-04

    [Abstract] Objective To investigate the effect of antioxidant N-acetylcysteine (NAC) on H9C2 cardiomyocyte injury induced by high-fat and high-glucose combined with hypoxia/reoxygenation and its effect on the expression of clock gene BMAL1. Methods H9C2 cells were divided into control group (N group)， high-fat and high-glucose combined with hypoxia/reoxygenation group (HFHG+H/R group)， high-fat and high-glucose combined with hypoxia/reoxygenation +NAC group (HFHG+H/R+NAC group). Cell viability was detected by CCK-8 method ......