汪白云 刘国良 朴成玉 陈巧云 陈景华 薛慧 刘斌

    [摘要] 目的 预测发现补阳还五汤防治阿尔茨海默病(AD)的作用靶标，探讨多成分-多靶点-多通路的协同作用机制。 方法 基于网络药理学方法针对补阳还五汤的方剂组成，采用多元网络药理学方法预测补阳还五汤的体内作用靶标，通过BATMAN-TCM等数据库对比补阳还五汤抗AD药物靶标，最终采用Cytoscape软件构建补阳还五汤成分-靶点-通路的网络。 结果 补阳还五汤中活性成分涉及多个与AD直接相关的体内靶标，如载脂蛋白E(APOE)等。同时涉及多个代谢通路，如甘油磷脂代谢、胆碱能突触、胆固醇代谢、AD通路等。 结论 补阳还五汤主要通过多成分协同起效，如1-十六碳烯对载脂蛋白E调节间接起到调控疏水性淀粉样β(Aβ)沉淀形成、藏花醛对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制作用等，进而延缓AD的发生发展。

    [关键词] 网络药理学;补阳还五汤;阿尔茨海默病;靶点;载脂蛋白E

    [中图分类号] R749? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-7210(2019)02(c)-0012-04

    [Abstract] Objective To predict and discover the target of Buyang Huanwu Decoction in the prevention and treatment of Alzheimer′s disease (AD) and explore the synergistic mechanism of multi-component， multi-target and multi-pathway. Methods According to the composition of Buyang Huanwu Decoction， the in vivo target of Buyang Huanwu Decoction was predicted by multiple network pharmacology methods. The target of anti-AD drug was selected by BATMAN-TCM and other databases. Finally， the components of Buyang Huanwu Decoction were constructed by Cycaptose software for target path network. Results The active components of Buyang Huanwu Decoction were involved in many targets directly related to Alzheimer′s disease ......