王东 李晶洁 吕书勤

    [摘要] 目的 采用代謝组学探究脾虚湿盛证肝癌患者发病机制及健脾化湿法干预的分子生物学效应。 方法 选取2017年6月～2019年6月新疆医科大学附属中医医院(以下简称“我院”)体检中心体检的50名平和质者作为空白组，选取我院收治的50例脾虚湿盛证肝癌患者作为对照组，50例服用四君子汤干预1个月的肝癌患者作为观察组。采用超高效液相色谱-高清质谱联用技术对三组入选者血浆样本进行代谢组学分析。 结果 在入选对象血液样品中共鉴定出谷胱甘肽、左旋谷氨酸等19个潜在生物标志物。与空白组比较，对照组中有6个表现为上升趋势，13个表现为下降趋势(P < 0.05或P < 0.01)。经四君子汤干预后，其中17个具有回调作用，调整差异有统计学意义(P < 0.05或P < 0.01);根据这17个回调生物标志物找到D-谷氨酰胺和谷氨酸代谢、牛磺酸和牛磺酸代谢等6条相关代谢通路。 结论 脾虚湿盛证肝癌患者体内存在氨基酸代谢、花生四稀酸代谢、脂肪酸代谢、谷胱甘肽代谢等紊乱，四君子汤可能通过调节以上相关代谢通路对脾虚湿盛证肝癌患者具有调节作用。

    [关键词] 肝癌;脾虚湿盛证;超高效液相色谱-高清质谱;血浆代谢组学

    [中图分类号] R246.5 ? ? ? ? ?[文献标识码] A ? ? ? ? ?[文章编号] 1673-7210(2020)04(c)-0145-04

    Study on plasma metabolomics of liver cancer patients with syndrome of dampness excessiveness due to spleen deficiency based on UPLC-HDMS

    WANG Dong ? LI Jingjie ? LYU Shuqin

    VIP Ward， Chinese Medicine Hospital of Xinjiang Medical University， Xinjiang Uygur Autonomous Region， Urumqi ? 830000， China

    [Abstract] Objective To study the pathogenesis of liver cancer with syndrome of dampness excessiveness due to spleen deficiency by metabonomics and the molecular biological effect of the intervention of invigorating spleen for eliminating dampness. Methods Fifty persons with peace quality of blank group were selected from the physical examination center of Chinese Medicine Hospital of Xinjiang Medical University (hereinafter referred to as “our hospital”) from June 2017 to June 2019. 50 cases of liver cancer patients with spleen deficiency and dampness syndrome in our hospital were taken as control group and 50 cases of liver cancer patients taking Sijunzi Decoction for one month were taken as observation group. Metabonomics analysis of plasma samples from three groups of participants was carried out by using the technology of ultra-high performance liquid chromatography-high definition mass spectrometry. Results Nineteen potential biomarkers such as glutathione and L-glutamic acid were identified in the blood samples of the selected subjects. Compared with blank group ......