采用“一测多评”法测定含绿原酸中药及制剂中绿原酸类成分的含量
张秋燕 张妮 张春礼 郭文全 崔翰明![](/images/paper/1673-7210/202025/02-4-l.jpg)
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[摘要] 目的 建立含綠原酸成分的中药及其制剂中所含的6种绿原酸“一测多评”(QAMS)的分析方法。 方法 以乙腈-0.4%磷酸为流动相梯度洗脱、柱温为25℃、流速为1.0 mL/min、检测波长为327 nm的高效液相色谱-二极管阵列检测器(HPLC-DAD)法,测定并计算以绿原酸为内参物的其他5种绿原酸类成分的相对校正因子;并考察相对校正因子的耐用性,确立以绿原酸为内参的QAMS法;分别采用已建立的QAMS分析方法和外标法计算含绿原酸中药(金银花、野菊花)及其滴眼剂中绿原酸类成分的含量,利用t检验和相对误差分析不同算法的含量差异,评价QAMS法的准确性;并采用相对保留时间方法对6种成分色谱峰进行准确定位。 结果 建立的以绿原酸为内参物的相对校正因子耐用性良好,采用2种方法计算10批次滴眼剂和3批次金银花及野菊花中绿原酸类成分的含量,两者相对误差范围为-3.32%~4.99%,差异无统计学意义(P > 0.05)。 结论 建立的QAMS法可同时用于金银花、野菊花中药材及其滴眼剂中6种绿原酸类成分的质量评价,具有快速、准确、方便等特点。
[关键词] 一测多评法;相对校正因子;滴眼剂;金银花;野菊花
[中图分类号] R284.2? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-7210(2020)09(a)-0010-05
[Abstract] Objective To establish the quantitative analysis of multi-components by single marker (QAMS) method for the analysis of six chlorogenic acids in traditional Chinese medicine and their preparations. Methods A high performance liquid chromatography-photodiode array detector (HPLC-DAD) method was developed with acetonitrile-0.4% phosphoric acid as mobile phase ingradient elution. The column temperature was 25℃. The flow rare was 1.0 mL/min and the detection wavelength was 327 nm. Then the relative correction factors among the other five chlorogenic acids were calculated ......
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