RP-HPLC法测定紫丁香叶中咖啡酸的含量
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【摘要】 目的 建立紫丁香叶中咖啡酸的含量测定方法。方法 采用RP-HPLC法测定紫丁香叶中咖啡酸的含量，色谱条件为：DiamonsilTM C18 (150 mm×4.6 mm，5 μm)分析柱，流动相：甲醇-0.1%磷酸水溶液(15∶85)，检测波长：323 nm，体积流量：1.0 ml/min，柱温：25℃。结果 咖啡酸的进样量在0.037 8～0.226 8 μg范围内与峰面积呈良好的线性关系(r=0.999 6)。平均回收率为98.4%，RSD为2.2%(n=5)；3批样品中咖啡酸的含量分别为 0.010 47、0.011 03、0.010 88 mg/g，RSD分别为2.0%、2.2%、1.6%。结论 本方法精密度高，分离度好，可用于紫丁香叶中咖啡酸的质量控制。
【关键词】紫丁香叶；咖啡酸；RP-HPLC
Determination of caffeic acid in Folium syringae
MI Bao-li,ZHANG Zhen-qiu.Liaoning University of Traditional Chinese Medicine,Dalian 116600,China
【Abstract】 Objective To establish the method for determining the content of caffeic acid in Folium syringae.Methods Determination of caffeic acid in Folium syringae by RP-HPLC,the chromatographic conditions were DiamonsilTM C18(150 mm×4.6 mm,5 μm)analytical column,mobile phase:methanol-0.1%phosphoric acid solution (15∶85),detection wavelength:323 nm,volume flow:1.0 ml/min,column temperature:25℃.Results A good liner range of caffeic acid was 0.037 8-0.226 8 μg(r=0.999 6),the average recovery was 98.4% and RSD was 2.2%(n=5);the caffeic acid contents of 3 batch samples were 0.010 47,0.011 03,0.010 88 mg/g,RSD were 2.0%,2.2%,1.6%.Conclusion This method with high precision and good resolution can be used for the quality control of Folium syringae. ......
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