葛根中葛根素的高效液相色谱分析(论著)

葛根中葛根素的高效液相色谱分析

http://www.100md.com 2008年5月1日《中国实用医药》 2008年第7期

     【摘要】目的测定不同产地葛根中葛根素的含量。方法用HPLC法测定，以MeOH-H2O-HAc(25∶75∶0.5)为流动相，250 nm为检测波长。结果葛根素在0.026～1.95 μg与峰面积呈良好线性关系(r=0.999 8),平均回收率为97.3%,RSD为1.35%(n=5)。结论该法简单、快速、准确,适用于葛根中葛根素含量的测定。

    【关键词】葛根;葛根素;HPLC

    Study ofpuerarin inredix puerariae by reversed-phase high performance liquid chromatography

    LI Hong-yu,DAI Shi-wen,SHOU Dan,et al.Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China

    【Abstract】 Objective Determination of Puerarin in the Redix Puerariae from different areas.Methods Puerarinwere determined by HPLC.The mobile phase was MeOH-H2O-HAc(25∶75∶0.5).Detection wavelength was at 250 nm.Results The Puerarin curve showed good linear correlation within 0.026-1.95 μg(r=0.999 8).The average recovery of Puerarin was 97.3% with RSD of 1.35%(n=5).Conclusion The method is simple and can be used to determine Puerarin with quick and accurate.

    【Key words】Redix puerariae; Puerarin;HPLC

    葛根为豆科植物野葛 Pueraria lobata (Willd.) Ohwi的干燥根,是常用中药材,具有解肌退热、生津、透疹、升阳止泻功效 ......

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