【摘要】 目的 建立测定糜蛋白酶中有机溶剂丙酮残留量的方法。方法 采用毛细管气相色谱法,色谱柱为Supelco 25301-U型石英毛细管柱，检测器为氢火焰离子化检测器，载气为高纯度氮气，水为溶解介质，检测了糜蛋白酶中丙酮的残留量。结果 丙酮在样品中达到完全分离,在1.59~25.50 ppm浓度范围内具有良好的线性,线性方程为A=306.0C+184.3，r=0.999 5，检测限为0.03 ppm,方法回收率为99.9%，RSD 为1.7%。结论 本法能快速准确的检测糜蛋白酶中有机溶剂丙酮残留量,可用于糜蛋白酶质量控制中的有机残留溶剂检查。

    【关键词】气相色谱法;糜蛋白酶;有机溶剂残留量

    Determination of acetone residue in chymotrypsin by capillary gas chromatography

    MIN Guang-qing.

    Deyang Sinozyme Pharmaceutical Co.,Ltd Sichuan，Deyang，618304，China

    【Abstract】 Objective To establish a method for determination acetone residue in chymotrypsin.Methods A capillary gas chromatography method was adopted：the column was Supelco 25301-U quartz capillary column,detector was FID,the carrier gas was Nitrogen gas.Water was solvent media.Results The linear range was 1.59-25.50 ppm(r=0.999 5) ......