高效液相色谱法同时测定复方丹参滴丸中丹参素和原儿茶醛
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【摘要】 目的 建立同时测定复方丹参滴丸中丹参素和原儿茶醛的高效液相色谱法。方法 选用C18柱，流动相为甲醇-水-冰醋酸(19∶80∶1)，检测波长为279 nm。 结果 丹参素和原儿茶醛分别在10~80 mg/L和2~16 mg/L线性范围关系良好,丹参素和原儿茶醛的平均回收率分别为99.4%和99.5%，RSD分别为1.6%和1.5%。结论 方法简便、灵敏、准确，样品处理简便易行，可作为复方丹参滴丸的质量控制方法。
【关键词】 复方丹参滴丸；丹参素；原儿茶醛；高效液相色谱法
Analysis of Danshensu and Protocatechuic Aldehyde in Compound Danshen Dropping Pill by High Performance Liquid Chromatoraphy
DING Lan，YI Ai-chun，SHI Zheng-rong.The First Hospital of Changsha，Changsha 410005，China
【Abstract】 Objective To separate and determine Dunshensu and protocatechuic aldehyde in Compound Danshen Dropping Pill.Methods The chromatographic conditions were as follow:Nucleosil C18 column.A mixture of methanol-water-acetic acid glacial(19∶80∶1)as the mobile phase with a flow rate of 1 ml per minute and the detection wavelength at 279 nm.Phydroxybenoic acid was used as internal standard.Results The calibration curves were linear in the range of 10~80 mg/L for Danshensu and 2~16 mg/L for protocatechuic aldehyde.The average recovery of Danshensu and protocatechuic aldehyde were 99.4%and 99.5% ......
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