三氟柳含量测定方法的建立
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【摘要】 目的 采用反相高效液相色谱(RP-HPLC)法测定三氟柳的含量。方法 采用DiamonsilTM(钻石)C18(250 mm×4.6 mm,5μm)色谱柱,流动相为0.1%磷酸水溶液-乙腈(50∶50),流速1.0 ml/min,检测波长224 nm。结果 在该色谱条件下,三氟柳在5.15~515 μg/ml范围内线性关系良好,r=0.9999。结论 该方法简便、准确、可行,可用于三氟柳的质量控制。
【关键词】 三氟柳;HPLC
Determination of the Content of Triflusal by RP - HPLC
WANG Bo.
Department of Pharmacy, the First People`s Hospital of Anqing,Anqing 246003,China
【Abstract】 Objective A reversed phase high performance liquid chromatography method for the determination of the content of Triflusal.Methods The analysis was performed on Diamonsil C18 reversed-phase analytical column(250 mm×4.6 mm,5 μm);the mobile phase consisted of a mixture of 0.1% phosphoric acid-acetonitrile (50∶50) at the flow rate was 1.0 ml/min;The ultraviolet detection wavelength was set at 224 nm ......
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