气腹对家兔肺损伤作用的初步研究(1)
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【摘要】 目的 探讨家兔气腹模型中不同气腹持续时间对肺的损伤作用。方法 以家兔为研究对象随机分为对照组(N组,假气腹组)、短时间气腹组(S组,气腹时间小于1 h)、长时间气腹组(L组,气腹时间大于3 h)。取各组血浆测血浆蛋白含量,分别取肺组织称取湿干重计算肺组织含水量、测定髓过氧化物酶(myleopemxidase,MPO)含量,取支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)离心后测定上清液蛋白含量和沉渣中白细胞,计算肺通透系数(lung permeability index,LPI)。结果 L组肺组织含水量及BALF中白细胞数大于S和N组(P<0.01),肺组织MPO活力明显高于S和N组。结论 大于3 h的持续气腹对肺组织造成了损伤,这种损伤,可能与中性粒细胞在肺内的募集、引发炎症反应等机制有关。
【关键词】气腹;肺损伤;肺通透系数;兔;中性粒细胞
The Effect of Pneumoperitoneum induced Lung Injury in Rabbits
BAO Yan-li,HUANG Zhong-hua,HU Jun,et al.Departmentofanesthesi ology,People’s Hospital of Guangxizhuang autonomous region,Nanning 530021,China
【Abstract】 Objective T oevaluat ethe effect of pneumoperitoneum induced lung injuryin rabbit.Methods 30rabbits were randomly assigned to following groups.The control group(N)was subjected to a sham pneumoperitoneum.The short time group(S)was subjected to 1 hour of pneumoperitoneum.The long time group(L)was subjected to 3 hours of pneumoperitoneum.After 20 min ofdesufflation subsequent to cessation of pneumoperitoneum,blood,lung,andbronchoalveolar lavage fluid(BALF)samples were obtained from every rabbit of the groups.white blood cell(WBC)count in BALF were detennined.The myleopemxidase(MPO)activity and wet to dry weight ratio(W/D)were also measured. Results Lung permeability index(LPI)and WBC count in BALF of group(L)are higher than the other t wo groups(P<0.01),The lung tissue MPO activity of group(L)is much higher than group(S)and group(N)(P<0.01).Conclusion Lung injuryinduced by more than3 hours of pneumoperitoneum is more effective than 1 hour of pneumoperitoneum.PMNs in lung tissue maybe responsible forthe inflammatory response induced bymore than3 hours of pneumoperitoneum rabbitlungs.
【Key words】Pneumoperitoneum; Lung injury; Lung permeability index;Rabbit;Polymorphonuclear leukocyte
基金项目:广西壮族自治区科技厅科学基金资助课题(项目编号:桂科青 0728041)
作者单位:530021广西壮族自治区人民医院麻醉科
CO2 气腹注入机体引起的腹内压增高可引起腹腔内血管血流阻力增高、灌注不足而导致内脏缺血,而放气减低了腹压,增加了脏器的灌注,因而在腹腔镜手术过程中出现了类似缺血/再灌注现象 [1]。目前认为缺血/再灌注现象造成脂质过氧化,从而引起自由基增加,并造成包括肺等组织器官的损害[2~4]。本实验以家兔为研究对象,初步探讨了气腹持续时间与肺损伤的关系。
1 资料与实验方法
1.1 实验对象 普通级健康家兔30只,月龄在3~5月,雌雄不拘,体重2 ......
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