游离HCV核心抗原检测对丙型肝炎早期筛选价值的研究(1)
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【摘要】 目的 分别检测游离丙肝病毒核心抗原(HCV-cAg)、抗体(HCV-Ab)及HCV RNA,研究游离丙肝病毒核心抗原检测早期筛选HCV感染的价值。方法 采用ELISA法测定丙肝病毒核心抗原和抗体，FQ-RT-PCR方法测定HCV RNA，对比300例HCV RNA阴性正常查体人群结果，筛选有危险因素人群HCV阳性者35例，研究丙肝病毒核心抗原测定的特异性和敏感性及缩短检测窗口期的价值。结果 体检人群300例HCV-Ab、HCV RNA均为阴性, HCV-cAg阴性299例(99.67%); 35例随访确诊HCV感染者初次HCV RNA阳性标本， HCV-cAg阳性33例(94.29%), HCV-cAg检测可有效缩短窗口期。结论 游离HCV核心抗原ELISA测定法具高度敏感性及特异性,可以明显缩短HCV窗口期,可作为HCV抗体检测的补充试验。
【关键词】 游离丙肝病毒核心抗原;丙肝病毒抗体; 反转录多聚酶链反应
Value study of Early Screening of Hepatitis C on Free HCV Core Antigen Detection
ZHANG LI,ZHANG WEN-Juan.
Medical college of Qingdao University,Shandong 266000,China
【Abstract】 Objective Hepatitis C virus core antigen (HCV-cAg) and antibody (HCV-Ab) and HCV RNA were detected to study the value of free hepatitis C virus core antigen when screening HCV early.Methods Hepatitis C virus core antigen and antibody measured by ELISA method. HCV RNA measured by FQ-RT-PCR method.Compared 300 patients with HCV RNA-negative results among normal physical examination, and we screened 35 HCV-positivecasesin the group with risk factors for hepatitis C virus. Through the above data we studied the specificity and sensitivity and the value of shorten the window period with determination of HCV core antigen. Results Results of HCV-Ab and HCV RNA in 300 physical examinations people were negative, and results of HCV-cAg in 299 patients (99.67%) were negative； To use diagnosis of follow-up methods we confirmed 35 cases of HCV infection. When first results of HCV RNA were positive, results of free HCV-cAg detection in 33 cases (94.29%) were positive.Detection of free HCV-cAg can effectively shorten the window period. Conclusion Free HCV core antigen ELISA assay with high sensitivity and specificity, it can significantly shorten the HCV window period, and it can be used as a supplementary test for HCV antibody test.
【Key words】 Free Hepatitis C virus core antigen; Anti-HCV; RT-RNA
丙型肝炎是严重威胁人类的传染病之一， 2008年数据表明，全世界丙型肝炎病毒感染的流行率约2.2~3.0%(1.30~1.70亿),其中还有许多地区还未相应的统计数据 [1]。现国内外HCV筛选主要应用抗HCV/EIA检测抗体，虽然第三代试验比第一代第二代试验提供更高的灵敏度和特异性 ......
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