Ad-Egr-hTrail联合放射线对脑胶质瘤细胞杀伤作用的实验性研究(1)
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【摘要】 目的 探讨重组腺病毒联合X射线对U251脑胶质瘤细胞的杀伤作用。方法 实验分6组：空白对照组(A组)，Ad-Egr-hTrail组(B组)，Ad-CMV-hTrail组(C组)，单独照射组(D组)，Ad-Egr-hTrail+照射组(E组)，Ad-CMV-hTrail+照射组(F组)。取对数生长期的U251细胞接种于96孔细胞培养板中， 48 h后重组腺病毒感染U25细胞，感染2 d后分别给予D、E、F组12 GyX射线一次性照射，照射后3 d，MTS法测定细胞抑制率。结果 ①B组与A组相比差异无统计学意义(P>0.05)，其余各实验组与对照组相比差异有统计学意义(P<0.01)。②两种腺病毒重组载体单独使用时，C组的抑制率明显高于B组(P<0.01)，两者联合放疗时，抑制率均明显提高(P<0.01)，E组是B组的7.43倍，F组是C组的1.84倍。③与D组相比，E组、F组的抑制率明显提高(P<0.01)，E组的放疗增敏比为1.38倍，F组的放疗增敏比为1.53倍。结论 ①Ad-Egr-hTrail具有很强的辐射诱导特性。②TRAIL基因具有辐射增敏作用。③两种腺病毒重组载体与放射治疗有正协同增敏作用.
【关键词】
Ad-Egr-hTrail; 基因-放射治疗;胶质瘤;辐射
Experimental study of Ad-Egr-hTrail combined with radiation on human glioma cell line
LIU Gui-hong, ZHANG Long-zheng, TANG Tian-you, et al. Affiliated Hospital of Xuzhou Medical Col lege， Xuzhou 221002， China
【Abstract】 Objective
To explore the synergistic induction of apoptosis by the combination of Ad-Egr-hTrail and radiation in U251 cell line.Methods The experiment was devided into six groups:control group(A group), Ad-Egr-hTrail group(B group), Ad-CMV-hTrail group(C group),radiation group(D group), Ad-Egr-hTrail combined with radiation group(E group), Ad-CMV-hTrailcombined with radiation group (F group).U251 cells were seeded in 96-well plates.Recombinant adenovirus and radiation were used on the U251 cells respectively and jointly.The inhibition rate was measured three days after radiation by MTS assay. Results ①Except B group,every experimental group was significantly different from control group (P<0.01).②When recombinant adenovirus vector was used alone， the inhibition rate of Ad-CMV-hTrail was significantly higher than that of Ad-Egr-hTrail(P<0.01). The inhibition rate of combined therapy was significantly higher than that of the respective application of gene therapy (P<0.01).The inhibition rate of E groupwas B group 7.14 times and F groupwas C group 1.84 times.③The inhibition rate of combined therapy group was significantly higher than that of radiation group(D group) (P<0 ......
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