FOXP3及调节性T细胞与支气管哮喘的相关性研究(1)
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【摘要】 目的 检测支气管哮喘患者外周血CD4+T细胞、CD4+CD25+T细胞、CD4+FOXP3+T细胞三类亚群，分析其在支气管哮喘患者的表达。方法 选择112例支气管哮喘患者和56例正常人作为对照，采用流式细胞术检侧患者外周血的CD4+ T细胞、CD4+CD25+T细胞、CD4+FOXP3+T细胞。结果 FOXP3主要表达在CD4+CD25+T细胞上，CD4+CD25+T细胞、CD4+FOXP3+T细胞与对照组相比均明显降低(P<0.01)，急性发作期哮喘患者外周血中CD4+CD25+T细胞、CD4+FOXP3+T细胞明显低于非急性发作期(P<0.01)，与疾病的活动性相关。结论 支气管哮喘患者存在明显的免疫功能失调，CD4+CD25+T细胞、CD4+FOXP3+T细胞在疾病活动中起着重要作用，是导致患者细胞免疫功能失调的重要原因。
【关键词】
支气管哮喘；FOXP3；调节性T细胞；免疫失调
The correlational study between FOXP3 and regulatory T cells with bronchial asthma
SUN Lailin, MA Jindong, WANG Huanxin, et al. Fangzi People,s Hospial,Sandong 261200,China
【Abstract】 Objective
This present study was designed to detect the CD4+, CD4+CD25+ and CD4+FoxP3+ T cells in bronchial asthma patents peripheral blood and analyze the expression levels of FoxP3 in three types of cells. Methods One hundred and twelve bronchial asthma patents were chosen for this study, with 56 normal people as the control. The CD4+, CD4+CD25+ and CD4+FoxP3+ T cells were detected by using the flow cytometry. Results FoxP3 was mainly expressed in CD4+CD25+T cells, whose expression level in CD4+CD25+ or CD4+FoxP3+T cells was significantly lower than control group(P<0.01). CD4+CD25+ and CD4+FoxP3+T cells in bronchial asthma patents with acuteoutbreak period were obviously lower than that in Non acute phase patents(P<0.01). Conclusion Bronchial asthma patents have obvious immune disorder. CD4+CD25+ and CD4+FoxP3+ T cells may play an important role in the progression of disease, whose low expression level may contribute to the immune disorder.
【Key words】
Bronchial asthma； FOXP3； Regulatory T cells； Immune disorders
支气管哮喘是一种慢性气道炎症性疾病，由肥大细胞、T细胞、嗜酸粒细胞等炎症细胞及细胞因子等组分参与引起，近年来支气管哮喘的患病率和死亡率有上升的趋势。迄今为止，支气管哮喘的发病机制尚不完全清楚，其发生多与过敏因素有关，而免疫因素也发挥着重要的作用。CD4+CD25+T淋巴细胞是人体重要的调节性T细胞，具有独特的作用方式和功能特征，近来逐渐引起了国内外学者的关注[1]。转录因子FOXP3主要表达在CD4+CD25+ T细胞上，在其细胞功能上发挥重要作用[2]，有关FOXP3及CD4+CD25+T细胞与支气管哮喘关系的研究国内报道较少，本文旨在探讨FOXP3及CD4+CD25+T细胞与支气管哮喘发病及疾病活动性的关系。
1 资料与方法
1.1 研究对象 支气管哮喘患者112例：为2007年5月至2011年9月我院门诊及住院患者，其中男66例，女56例，年龄16～76岁，平均年龄(41.7±16.8)岁。所有病例均符合2003年中华医学会呼吸病学分会哮喘学组制定的支气管哮喘防治指南中规定的诊断标准[3] ......
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