HPLC法测定苦瓜清脂素胶囊中盐酸西布曲明

http://www.100md.com 2012年7月15日《中国实用医药》 2012年第20期

     【摘要】建立高效液相色谱法测定盐酸西布曲明的含量。色谱柱为Venusil MP C18, 流动相为含01%甲酸的20 mmol/L醋酸铵甲醇(35∶ 65)。检测波长为224 nm，流速为10 ml/min。盐酸西布曲明在00225～0225 mg/ml范围内与峰面积线性关系良好(r=09998)；平均回收率为9887%，RSD为065%(n=6)。本方法简便、准确、灵敏、重现性好，可作为盐酸西布曲明的含量测定方法。

    【关键词】高效液相色谱法；苦瓜清脂素胶囊；盐酸西布曲明

    Determination of Sibutramine in Kuguaqingzhisu capsule by HPLC JIANG Jiaxin,ZI Yang.Department of Bidogical Resource,Lushan Branch Institate of Guangxi Technical College,Liuzhou 545616,China

    【Abstract】 To establish a method for the determination of Sibutramine in kuguaqingzhisu capsule by HPLC HPLC was used Venusil MP C18 column, mobile phase was methanol20 mmol/L ammonium acetate (01%formic acid) (35:65),The flow rate was 10 ml/min with detection wavelength at 224nm  The linear range of sibutramine was from 00225～0225 mg/ml (r=09998) The average recoverery was 9887% and RSD was 065%(n=6) The method is simple,accurate and suitable It can be used for the determination of Sibutramine in kuguaqingzhisu capsule. ......

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