复方倍他米松抑制兔耳瘢痕增生与氧自由基作用的初步研究
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[摘要] 目的 了解复方倍他米松局部注射对兔耳增生性瘢痕组织中丙二醛含量的影响，并探讨其与氧自由基的关系。方法 新西兰兔14只制作兔耳增生性瘢痕模型，正常兔耳皮肤组织标本8例；兔耳增生性瘢痕组织标本28例，随机分为复方倍他米松组(10例)、生理盐水组(10例)、空白对照组(8例)。术后6周予复方倍他米松(40 mg/ mL)分点注射于瘢痕样组织内，每处2~3点，总量0.3~0.4 mL，每周1次，3次为一疗程。术后9周取材，计数成纤维细胞，并测量瘢痕的相对增生厚度，测定丙二醛含量变化。结果 ①大体形态学变化：复方倍他米松部治疗3周后，瘢痕颜色接近兔耳的正常肤色，略高出皮面，表面工整，触之质软。②组织学变化：与其他三组比较，复方倍他米松组胶原纤维多为平行排列，数量减少。③成纤维细胞密度与瘢痕增生指数变化：与其他三组比较，复方倍他米松组成纤维细胞密度增高(P<0.05)，④丙二醛含量变化：与其他三组比较，复方倍他米松组丙二醛含量明显增高(P<0.05)； 结论 复方倍他米松局部注射兔耳创面引起兔耳增生性瘢痕组织中氧自由基水平升高。
[关键词] 增生性瘢痕；复方倍他米松；氧自由基；细胞凋亡；成纤维细胞
[中图分类号] R782.2[文献标识码] A[文章编号]1673-9701(2011)21-09-03
Inhibitory Effect of Triamcinolone Acetonide on Hypertrophic Scar and Oxygen Free Radicals in Rabbits
WU Xin min1LI Xia2 ZHOU Qinsheng1 MA Long3BAI Jing1 TAN Ge1
1.Department of Plastic Surgery, Fifth Affiliated Hospital,Xinjiang Medical University，Urumqi 830011，China; 2. Department of Anesthesiology, Fifth Affiliated Hospital,Xinjiang Medical University，Urumqi 830011，China; 3.The Reshaping Burns the Traumatology Department，Xinjiang Production and Construction Corps Hospital，Urumqi 830011，China
[Abstract] Objective To investigate the inhibitory effect of triamcinolone acetonide on hypertrophic scar and oxygen free radicals in rabbits. Methods Eighteen New Zealand rabbits were used. 14 rabbits were selected randomly to construct animal models of hypertrophic scar on the ears. Another 4 rabbits(8 ears)were used as controls. 6 weeks after operation，the hypertrophic scar on the ears were randomly divided to receive intra-lesion injection of TA(n=10)，or normal saline(n=10)，or nothing( n=8，sham group).9 weeks after operation，scar specimens were taken for scar thickness measurement，fibroblast counting under microscopy and MDA content detection by spectrophotometric method. Results ①3 weeks after TA treatment，the scar became very thin and soft with a similar color to normal skin and a smooth surface；②Histologic study showed the collagen fibers in TA group were reduced markedly and arranged parallelly；③Compared with normal skin ......
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