柴胡注射液对梗阻性黄疸大鼠肝损伤的保护作用(1)
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[摘要] 目的 探讨柴胡注射液对梗阻性黄疸大鼠肝损伤的保护作用。方法 SD大鼠60只随机分为3组：假手术组、梗阻性黄疸模型组、柴胡注射液干预组；后两组采用胆总管结扎法制作梗阻性黄疸模型，术后第1天起分别给药，并于术后第7、14天分别测定不同组别大鼠血清ALT、AST、TBIL、DBIL等指标。结果 柴胡注射液干预组术后第7、14天大鼠血清ALT、AST活性及TBIL、DBIL含量明显下降，与梗阻性黄疸模型组比较，差异有统计学意义(P<0.05)。结论 柴胡注射液对梗阻性黄疸大鼠肝损伤具有一定的保护作用。
[关键词] 柴胡注射液；梗阻性黄疸；肝损伤
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701(2011)23-12-02
Protective Effects of Bupleurum Injection on Hepatic Injury Induced by Obstructive Jaundice in Rats
JIN Jianwei1 LIU Zhong2
1.Department of Surgery，Xidian Town Hospital of Ninghai County of Ningbo City in Zhejiang Province，Ninghai 315132，China；2.Department of General Surgery，Affiliated Hospital of Ningbo University，Zhejiang Province，Ningbo 315020，China
[Abstract] Objective To explore the protective effects of Bupleurum Injection on hepatic injury induced by obstructive jaundice in rats. Methods Sixty rats were divided into 3 groups，the sham operation group，the bile duct ligation group and Bupleurum Injection group. Obstructive jaundice were made in rats by double-ligating bile duct in the bile duct ligation group and Bupleurum Injection group. Three groups of rats were sacrificed after 7 and 14 days of postoperation respectively. The levels of serum ALT，AST，TBIL and DBIL were tested. Results The group of Bupleurum Injection could decreased the levels of serum ALT，AST，TBIL and DBIL. Compared with model control group，there was significant difference (P<0.05). Conclusion Bupleurum Injection has protective effects on hepatic injury induced by obstructive jaundice in rats.
[Key words] Bupleurum Injection；Obstructive jaundice；Hepatic injury
梗阻性黄疸(obstructive jaundice，OJ)是指由肿瘤、结石、炎症等多种原因导致的肝内外胆管机械性梗阻所致的黄疸，肝胆组织受损是其主要病理学表现之一[1]。2009年1月～2010年10月我们用胆总管结扎法制造大鼠梗阻性黄疸病理模型，探讨柴胡注射液对梗阻性黄疸大鼠肝损伤的保护作用，为开发柴胡注射液临床新用途提供基础试验资料依据。
1 材料与方法
1.1 材料
1.1.1 研究对象 SD大鼠，雌雄各半，体重(220±20)g，由浙江大学医学动物试验中心提供，动物合格证号：SCXK(浙)2007-0005。
1.1.2 药物与试剂 柴胡注射液(神威药业有限公司)；丙氨酸氨基转氨酶(ALT)、天门冬氨酸氨基转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)试剂盒(南京建成生物工程研究所)。
1.2 方法
1.2.1 动物分组[2] 取SD大鼠60只，适应性喂养3d后随机分为3组，即假手术组、梗阻性黄疸模型组、柴胡注射液干预组，每组20只。造模手术后再将各组动物随机分为第7、14天两个时相组 ......
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